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1

Evolution of eukaryotic chromosomes ? from databases to computer modeling

100%
Mackiewicz D., Waga W., Cebrat S.
Biotechnologia
|
2010
|
issue 4
131-145
EN
Computer simulations of chromosomes' and genomes' evolution suggest that the genes located on relatively large and densely packed chromosomes should be grouped in clusters. Clusters located on homologous chromosomes may complement their defects or they may co-operate providing selective advantage to their hosts. Since recombination inside clusters is harmful, selection leads to the uneven distribution of recombination events along chromosomes - relatively high recombination in the subtelomeric regions and low recombination in the central regions of chromosomes. Uneven distribution of recombinations enables sympatric speciation which can not be predicted by the mean field theories of evolution. Further studies of chromosome evolution require more precise data (the best ? full sequences) of many closely related genomes belonging to the same species.
2

Improvement of beta-galactosidase properties and conditions for its microbial biosynthesis

88%
Bednarski W., Kowalewska-Piontas J.
Biotechnologia
|
2000
|
issue 2
131-141
EN
We present examples of genetic modification of microorganisms capable of beta-galactosidase synthesis. The technological characteristics as termostability, high activity in a low temperature is improved. We also describe intensification of beta-galactosidase secretion to the medium.
3

Genetic control of hordein polypeptides in kernels of spring barley (Hordeum vulgare L.) high-lysine mutant C-67-7

88%
Kapala A., Patyna H.
Genetica Polonica
|
1993
|
vol. 34
|
issue 2
133-138
EN
The subject of the studies were F1 and F2 hybrids originating from reciprocal crosses of the highlysine mutant C-67-7 and cultivar Miranda, the forms with different hordein polypeptide spectra. The method of SDS gel electrophoresis was used for an analysis of the main hordein fractions, hordein-1 and hordein-2, from single kernels of barley. It was found that polypeptide spectra of hordein-1 and hordein-2 of the high lysine mutant C-68-7 are determined by codominant alleles at two loci, which are moderately linked.
4

Generation of selectable marker-free transgenic plants

75%
Zuzga S., Burza W., Malepszy S.
Biotechnologia
|
2003
|
issue 4
32-46
EN
Since the first reports of successful plant transformation appeared, there have been steady improvements of the transformation methods. Nowadays, transgenic plants without the incorporation of selection genes for antibiotic or herbicide resistance would be the only ones acceptable to the public, so elimination of these genes from transgenic crops prior to their field release and commercialization seems inevitable. Several strategies have been developed to generate marker-free transgenic plants, including: positive selection, alternative marker genes, co-transformation, site-specific recombination, transpozon-mediated approaches and intrachromosomal homologous recombination. The efficiency of these methods is various as comparing to the traditional marker assisted selection - higher in case of alternative procedures and lower in others.
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