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Effect of Protein Extract of Mangifera indica Pollen on the Erythrocytes (Red Blood Cell) of Albino Rats

100%

Ezebo R. O., Omumuabuike J. N., Esimai B. G., Ilouno E. E., Obieze O. U., Obienyem J. N., Uba C. A.

World News of Natural Sciences

2021

vol. 36

114-124

This study was carried out to show the effect of the protein extract of Mangifera indica on the red blood cell (erythrocytes) of albino rats. The Mangifera protein was extracted using 500ml of 0.02m (20mM) phosphate- buffered saline (PBS), at pH 7.4, in a large beaker (stirred with a magnetic stirrer for 3 hours at room temperature). The crude extract was saturated to 60% by adding solid ammonium sulphate under constant gentle attiring, and then stored in a refrigerator for 6 hours. Sixteen male albino rats obtained from animal house in Faculty of Biological Science, University of Nigeria Nsukka was used for the study. They were divided into two groups of eight. One group was labeled the experimental group and the other control. The extract was administered to the experimental rats intra nasally for a period of seven (7) days. Data were expressed as mean ± standard error. Means were separated using Duncan’s New Multiple Range Test (DNMRT). Blood samples were collected via the orbital plexus of rats to determine the effect of the extract on red blood cell (erythrocytes). The present study demonstrated that the extracted pollen of Mangifera indica had no allergic effect on ratsand so would need to be further investigated.

Pollen analysis and heavy metals detection in honey samples from southern Nigeria

67%

Adekanmbi O. H., Walter O. J., Ikegbunam N. C.

vol. 26

176-190

Pollen analytical study of two locally produced honey samples collected from Akwa Ibom and Cross River State, Southern Nigeria were carried out in order to ascertain the preferentially foraged plants by honey bees, as well as the concentration of heavy metals. Samples were subjected to standard laboratory procedures using an acetolysis mixture (pollen analysis) and atomic absorption spectrometry (heavy metals). The results from the analysis revealed 32 taxa belonging to 17 botanical families. The number of pollen grains in the honey samples varied significantly (between 4,887 - 12,755 pollen grains), indicating their richness in pollen grains. 12 elements - Ni, Cu, Zn, Se, Br, K, Ca, Ti, Cr, Mn, Rb, and Fe were detected. Potassium had the highest concentration, followed by iron, calcium, titanium, zinc, copper, chromium, nickel, among others. What is more, in this study, some important honey plants: Spondias mombin, Daniella oliveri, Manihot esculenta, Treculia africana, Syzigium guineensis, Diospyros mespiliformis, Parkia biglobosa, Terminalia superba, Senna hirsuta, among others were found to be predominant and have been identified to be characteristic of the vegetation typical of Southern Nigeria. With regard to honey, this study gives an indication of the geographical and botanical origins, as well as types, source, and degree of contamination, and also an overall measure of honey purity. Such information, when displayed, can help consumers make informed decision when purchasing honey and will also help beekeepers to avoid possible contamination. Moreover, it will assist regulatory agencies in taking proper measures for environmental and consumer protection, since the concentration of heavy metals in honey is influenced by environmental pollution.

Frequency of allergic sensitivity to local herbs pollen among medical students in East of Iran by use of skin prick test

67%

Chamani S., Mahmoudzadeh S., Rezapour H., Fereidouni M.

Alergia Astma Immunologia - przegląd kliniczny

2021

vol. 26

issue 1

27-35

Wprowadzenie: Choroby alergiczne stanowią jeden z najbardziej powszechnych problemów zdrowotnych, wpływają na jakość życia pacjenta i stanowią poważne obciążenie ekonomiczne. Ziarna pyłków roślin są najistotniejszym czynnikiem wywołującym objawy alergii. Znajomość siły alergizacji pyłków roślin występujących na danym obszarze ma zasadnicze znaczenie dla zapobiegania objawom alergii. Cel pracy: Celem badania była ocena występowania uczuleń na pyłki lokalnych roślin wśród studentów medycyny we wschodnim Iranie za pomocą testów nakłucia naskórka (prick test). Materiał i metody: Typy uczulających pyłków dobrane zostały w oparciu o analizę danych geoklimatycznych. Do badań użyto dziesięć ziaren pyłków roślin typowych dla regionu, w tym drzewa Eucalyptus, drzewa Helianthus, kwiatów Rosa damascene i Jasminum sp, drzewa Chinaberry (Melia azedarach), drzewa granatu, czarnych oliwek, drzewa pistacjowego, palm i kwiatów Crambe cordifolia, a także siedem standardowych, komercyjne dostępnych zestawów ekstraktów, w tym mieszanek traw, drzew i pyłków Fraxinus, Amaranthus retroflexus, Cypress, Betula i Prosopis. Po oczyszczeniu preparatów, przygotowywano wodne roztwory ekstraktów pyłków. Badania przeprowadzano u ochotników metodą nakłucia naskórka (prick test) ze wszystkimi dziesięcioma ekstraktami lokalnych roślin oraz kilkoma ekstraktami komercyjnymi. Badanie zostało zatwierdzone przez komisję ds. etyki Uniwersytetu Medycznego Birjand, a wszyscy uczestnicy podpisali formularz zgody. Wyniki: W badaniu wzięło udział 112 studentów medycyny (średni wiek: 22,29, zakres: 19–34 lata, stosunek M / K: 1,1). Ogólna częstość występowania uczulenia na pyłki co najmniej jednego z ekstraktów wynosiła 80,59%. W przypadku pyłków roślin lokalnych największe odczyny skórne stwierdzono dla pyłków drzewa Eucalyptus, kwiatów Rosa damascene i Jasminum sp (odpowiednio 59,3%, 53,1% i 50,0%). W odniesieniu do ekstraktów dostępnych komercyjnie najczęściej stwierdzano uczulenia na Amaranthus retroflexus i mieszaninę pyłków drzew (odpowiednio 66,6% i 45,7%). Wnioski: Wyniki badań wskazują na wysoką siłę alergizującą niektórych pospolitych roślin regionalnych, w tym kwiatu róży, drzewa eukaliptusa i kwiatu jaśminu.

Background: Allergic disorders are among the most common health problems, have a high impact on patients’ quality of life and constitute a significant economic burden. Pollen grains are the most significant trigger of allergic symptoms. Knowing pollen allergy potency of common plants in each area is essential for preventing allergic symptoms. Objective: This study aims to evaluate allergic sensitivity to local plant pollen among medical students in East of Iran using skin prick test Methods: Types of allergenic pollen grains were determined based on geoclimatic factors. Ten different pollen grains from common regional plants, including Eucalyptus tree, Helianthus trees, Rosa damascene flowers, Jasminum sp flowers, Chinaberry trees (Melia azedarach), pomegranate trees, black olive trees, pistachio trees, Palm trees, and Crambe cordifolia flowers, as well as seven standard commercial extracts including grass mixture, tree mixture, Fraxinus, Amaranthus retroflexus, Cypress, Betula, and Prosopis pollens were collected. After purification, the aqueous extract was prepared from each pollen. Skin prick test with ten domestic extracts and some commercial extracts was performed on the participants. The ethics committee of Birjand University of Medical Sciences approved the study and all participants signed consent form. Results: One hundred and twelve volunteer medical students (mean age: 22.29 range: 19 to 34 years, M/F ratio:1.1) enrolled in this study. The overall frequency of sensitivity to any allergenic extracts was reported at 80.59%. In domestic pollen, the highest skin sensitivity rate was for Eucalyptus pollen, Rosa damascene flowers pollen, and Jasminum sp pollen (59.3%, 53.1%, and 50.0%, respectively). In the case of commercial extract, Amaranthus retroflexus and the tree mixture pollens were the most common (66.6% and 45.7%, respectively). Conclusion: This study showed high allergic potency of some common regional plants, including Rose flower, Eucalyptus tree, and Jasminum sp flower.

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2 World News of Natural Sciences

1 Alergia Astma Immunologia - przegląd kliniczny

2 2021

1 2019

1 Adekanmbi O. H.

1 Chamani S.

1 Esimai B. G.

1 Ezebo R. O.

1 Fereidouni M.

1 Ikegbunam N. C.

1 Ilouno E. E.

1 Mahmoudzadeh S.

1 Obienyem J. N.

1 Obieze O. U.

1 Omumuabuike J. N.

1 Rezapour H.

1 Uba C. A.

1 Walter O. J.

Search results

Effect of Protein Extract of Mangifera indica Pollen on the Erythrocytes (Red Blood Cell) of Albino Rats

Pollen analysis and heavy metals detection in honey samples from southern Nigeria

Frequency of allergic sensitivity to local herbs pollen among medical students in East of Iran by use of skin prick test