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Temperature effect on growth, and selected parameters of Phaeodactylum tricornutum in batch cultures

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Bojko M., Brzostowska K., Kuczyńska P., Latowski D., Olchawa-Pajor M., Krzeszowiec W., Waloszek A., Strzałka K.
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2013
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vol. 60
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issue 4
861-864
EN
The effect of optimal and stress temperatures on the growth kinetics of the Phaeodactylum tricornutum CCAP/1055/1 strain (a model diatom with a known genome sequence) in batch cultures was examined. The analysis of the obtained results showed two phases of culture growth. There were significant positive correlations between OD increase of chlorophyll a chlorophyll c and protein concentration at different temperatures. The Fv/Fm parameter achieved a maximum level on the 6th or 7th day and then decreased to the values registered on the first day of observation. Genetic material undergoes gradual degradation 10 days after inoculation. The size of the cells was invariable.
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Column chromatography as a useful step in purification of diatom pigments

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Tokarek W., Listwan S., Pagacz J., Leśniak P., Latowski D.
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2016
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vol. 63
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issue 3
443-447
EN
Fucoxanthin, diadinoxanthin and diatoxanthin are carotenoids found in brown algae and most other heterokonts. These pigments are involved in photosynthetic and photoprotective reactions, and they have many potential health benefits. They can be extracted from diatom Phaeodactylum tricornutum by sonication, extraction with chloroform : methanol and preparative thin layer chromatography. We assessed the utility of an additional column chromatography step in purification of these pigments. This novel addition to the isolation protocol increased the purity of fucoxanthin and allowed for concentration of diadinoxanthin and diatoxanthin before HPLC separation. The enhanced protocol is useful for obtaining high purity pigments for biochemical studies.
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