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Diversity, phenology and biological spectrum of tree flora in upper Tanawal, district Mansehra, KP, Pakistan

100%

Farooq M., Anjum W., Hussain M., Saqib Z., Shah A. H., Khan K. R., Nazir J., Gul S.

World News of Natural Sciences

2018

vol. 16

86-96

Upper Tanawallies is in the middle of the Western part of Hazara division, and includes the remote areas of four districts i.e. Mansehra, Abbottabad, Haripur and Tor-Ghar, having latitude 34°.34.40´ N to 34°.48.88´ N and 72°.84.27´ E to 73°.10.50´ E longitude. This area is loaded with plant diversity, and tree species in this area vary in their life form, leaf spectra and phenological behavior. There is no prior record of plants diversity in the area, hence, this study was conducted to explore the tree diversity, life form, leaf spectrum and phenology of the tree flora. A total 127 sampling stands (10 x 10 m2) were put into place in different locations in Upper Tanawallies to collect field data using the quadrat method. As an outcome, we learned that the region hosts 53 different tree species of 39 genera belonging to 25 tree families. The biological spectra of the trees were constructed according to Raunkiaer (1934). Results showed that Mesophanerophytes was the dominant life form class, contributing 34 (64%) of all tree species encountered, while Mesophyll and Microphyll was the dominant leaf size spectrum classes, each contributing to a count of 18 (34%) of all tree species. Frequent field visits were also carried out during the flowering and fruiting seasons in 2016/17. The result indicates that most tree species of the area show flowering during April-May (32%) while, maximum fruiting were recorded in June-July (36%). Our study concludes that anthropogenic activities on these forests should be reduced to overcome deforestation. This work will be the baseline for new research in the study area.

First report of successful Naegleria detection from environmental resources of some selected areas of Rawlakot, Azad Jammu and Kashmir, Pakistan

86%

Akbar A., Hameed A., Aloufi A. S., Almutairi M. M., Tanveer T., Matin A.

vol. 60

37-45

Naegleria belongs to the free-living amoeba family and is well-known as a human pathogen. It is recognized as etiological agent of primary amoebic meningoencephalitis involving central nervous system which always leads to death. To date, there is not a single report demonstrating Naegleria isolation and identification from environmental sources of Rawlakot, Azad Jammu and Kashmir Pakistan, and thus the aim of this study. Naegleria was isolated on non-nutrient agar plates seeded with heat killed E. coli and confirmed by morphological properties of the both stages of cyst or trophozoites. Furthermore, PCR was conducted along with direct sequencing of the PCR product for molecular identification. PCR and sequencing data verified the amplification of Naegleria sp. (07) and Vahlkampfia sp. (01) from both water and soil samples. Interestingly two species were successfully isolated and cultured on both 30 and 45°C. To the best of our knowledge this is the first report demonstrating the Naegleria isolation and molecular characterization from environmental sources of Rawlakot, Azad Jammu and Kashmir, Pakistan. The author is anxious for further evaluation of the pathogenic potential of the identified species and explores drinking water across Pakistan to investigate its quality and frequency of FLA, which might be a possible human hazard in future.

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1 Acta Protozoologica

1 World News of Natural Sciences

1 2022

1 2018

1 Akbar A.

1 Almutairi M. M.

1 Aloufi A. S.

1 Anjum W.

1 Farooq M.

1 Gul S.

1 Hameed A.

1 Hussain M.

1 Khan K. R.

1 Matin A.

1 Nazir J.

1 Saqib Z.

1 Shah A. H.

1 Tanveer T.

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Diversity, phenology and biological spectrum of tree flora in upper Tanawal, district Mansehra, KP, Pakistan

First report of successful Naegleria detection from environmental resources of some selected areas of Rawlakot, Azad Jammu and Kashmir, Pakistan