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EN
The presence and distribution of CRISPR (clustered regularly interspaced short palindrome repeat) elements in the archaeal order Thermococcales were analyzed. Four complete genome sequences from the species Pyrococcus abyssi, P. furiosus, P. horikoshii, and Thermococcus kodakaraensis were studied. A fragment of the genome of P. furiosus was flanked by CRISPR elements upstream and by a single element downstream. The composition of the gene sequences contained in this genome fragment (positions 699013 to 855319) showed significant differences from the other genes in the P. furiosus genome. Differences were observed in the GC content at the third codon positions and the frequency of codon usage between the genes located in the analyzed fragment and the other genes in the P. furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea.
EN
Some enzymes from extreme thermophiles and hiperthermophiles have half-lives up to 13h at ,temperatures above 100?C. They are suitable for starch and whey processing, oil mining, PCR techni and oligosaccharides synthesis. The differences in structure and function between these very stable and the prevailing less stable enzyme forms are relatively small and are comparable with those differences found among enzymes of similar stability. The relationship of both conformational stability and enzymatic activity with protein flexibility suggests that stability at temperatures far above those which are optimum for the growth of microorganism is rather unlikely.
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