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1

Somatic hybridization between wild and cultivated Solanum species

100%
Wielgat B., Wasilewska L. D.
Biotechnologia
|
2001
|
issue 2
9-15
EN
Many of the wild species of Solanum possess high level of disease resistance to viruses, bacteria and pathogenic fungi that could be useful in potato breeding. Somatic hybridization technique enables introgression of those traits from related wild species into cultivated potato and appears to be very a useful means by which the sexual incompatibilities might be bypassed. The article discusses some aspects of potato somatic hybridization.
2

Wheat tissue culture - a reviev

88%
Menke-Mielczarek I.
Biotechnologia
|
1997
|
issue 2
29-45
EN
Based on the available literature, this article describes the advances made in cell culture of wheat. The importance of the age and physiological stage of the explant is discussed. The influence of the genotype is observed. The role of the components of the induction medium and in particular the role of auxin and kinetin, is investigated. The development of off-white callus and long-term culture of this callus facilitates the establishment of suspension culture - a source of totipotent protoplasts. This paper also focuses on the techniques which are used to introduce genes into wheat plants and on the somaclonal variation that occurs in a population of plants regenerated after tissue culture.
3

Cellular aspects of regeneration in protoplast culture of yellow lupin (Lupinus luteus L.)

88%
Wiszniewska A., Pindel A.
Biotechnologia
|
2010
|
issue 2
18-22
EN
Yellow lupin (Lupinus luteus), like many other Fabaceae species, exhibits strong recalcitrance to in vitro conditions of protoplast culture, where regeneration capacity is extremely limited, and only incipient mitoses are observed. In case of the restricted morphogenetic potential of yellow lupin protoplasts, special attention should be paid to the cellular mechanisms that control gaining of totipotency in culture. These are especially: the structure and functioning of new cell wall, elements of cytoskeleton, as well as some cell components of signaling properties. Detailed investigation on these structures and their behaviour in culture conditions may contribute to the understanding and bypassing of the recalcitrance problem in yellow lupin.
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