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1

Lipopolysaccharides of the genus Proteus

100%
Sidorczyk Z.
Postępy Higieny i Medycyny Doświadczalnej
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1996
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vol. 50
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issue 5
401-417
EN
In this review up-to-date knowledge on chemical structure and epitope specificity of Proteus lipopolysaccharides is summarized, with special emphasis to P. penneri LPS
2

Classification of Proteus mirabilis TG 115 and CCUG 10701 into the Proteus O23 serogroup based on chemical and serological studies of O-polysaccharides

100%
Zablotni A., Perepelov A. V., Kolodziejska K., Zych K., Knirel Y. A., Sidorczyk Z.
Archivum Immunologiae et Therapiae Experimentalis
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2006
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vol. 54
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issue 6
411-417
EN
Introduction: Bacteria of the genus Proteus are a common cause of urinary tract infections. The O-polysaccharide (OPS) chain of their lipopolysaccharide (LPS) defines the serological specificity of strains. Based on the OPS structures and the immunospecificity of the LPS, Proteus strains have been classified into 74 O-serogroups. Materials and Methods: The OPS of P. mirabilis TG 115 was obtained by mild acid degradation of the LPS and studied by 1H and 13C nuclear magnetic resonance spectroscopy. Antisera were raised by immunization of rabbits with heat-killed bacteria. Serological studies were performed using enzyme immunosorbent assay, passive immunohemolysis, inhibition experiments, absorption of O-antisera, and Western blot.Results: The following structure of the P. mirabilis TG 115 OPS was established: 2)--D-GalpA-(13)--D-GalpNAc-(14)--D-GalpA-(13)--D-GlcpNAc-(1 The same structure has been reported previously for the O-polysaccharides of P. mirabilis CCUG 10701 (O74) and P. mirabilis 41/57 (O23), except that they contain O-acetyl groups in non-stoichiometric quantities. Serological studies showed the antigenic identity of the three strains and their close serological relatedness to P. vulgaris 44/57. Conclusions: Based on the OPS structures and serological data, it is suggested to classify P. mirabilis 41/57, TG 115, and CCUG 10701 into one subgroup and P. mirabilis 42/57 and P. vulgaris 43/57 and 44/57 into another subgroup of the Proteus O23 serogroup.
3

Serological classification and epitope specificity of Proteus vulgaris TG 251 from Proteus serogroup O65

88%
Zych K., Kolodziejska K., Drzewiecka D., Perepelov A. V., Knirel Y. A., Sidorczyk Z.
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2007
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vol. 55
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issue 3
187-191
EN
Introduction: Proteus rods are currently subdivided into five named species, i.e. Proteus mirabilis, P. vulgaris, P. penneri, P. hauseri, and P. myxofaciens, and three unnamed Proteus genomospecies 4 to 6. Based on the serospecificity of the lipopolysaccharide (LPS; O-antigen), strains of P. mirabilis and P. vulgaris were divided into 49 O-serogroups and 11 additional O-serogroups were proposed later. About 15 further O-serogroups have been proposed for the third medically important species, P. penneri. Here the serological classification of P. vulgaris strain TG 251, which does not belong to these serogroups, is reported. Serological investigations also allowed characterization of the epitope specificity of its LPS. Materials and Methods: Purified LPSs from five Proteus strains were used as antigens in enzyme immunosorbent assay (EIA), SDS/PAGE, and Western blot and alkali-treated LPSs in the passive immunohemolysis (PIH) test, inhibition of PIH and EIA, and absorption of the rabbit polyclonal O-antisera with the respective LPS. Results: The serological studies of P. vulgaris TG 251 LPS indicated the identity of its O-polysaccharide with that of P. penneri O65. The antibody specificities of P. vulgaris TG 251 and P. penneri O65 O-antisera, were described. Conclusions: P. vulgaris TG 251 was classified to the Proteus O65 serogroup. Two disaccharide-associated epitopes present in P. vulgaris TG 251 and P. penneri O65 LPSs are suggested to be responsible for cross-reactions with three heterologous Proteus strains.
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