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The application of the tissue cultures in protection of rare and threatened species of ferns from Lower Silesia

100%
Marsza? J., Kromer K.
Biotechnologia
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2004
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issue 2
237-243
EN
One of the possibilities of the conservation biology is the in vitro culture method. The usefulness of in vitro culture methods of spore planting and prothallium storage of rare, threatened and protected fern species were tested. Spors of Asplenium adulterinum, A. cuneifolium, A. septentrionale and Polypodium vulgare were collected from sporophytes growing in the natural habitats of Lower Silesia area in 1996. The collected spores were disinfected and the aseptic spores were cultured in flasks containing 1/4 MS solid medium. After three to eight months, the spores germinated, giving rise to a filamentous gametophytes. Six to eight months later, spotophytes could be observed in these cultures. The in vitro method gives the possibility to investigate the special characteristics of life cycle and breeding system of fern species. It is possible to collect fern spores from natural habitats and use them to obtain populations, which will allow to preserve the fern population in cultivation in the Botanical Garden of the Wroclaw University.
2
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Propagation of EEG activity during finger movement and its imagination

75%
Kus R., Ginter J. S., Blinowska K. J.
Acta Neurobiologiae Experimentalis
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2006
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vol. 66
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issue 3
195-206
EN
We investigated the pattern of EEG activity propagation in the beta and gamma band during a finger movement experiment and imagination of that task. The data were analyzed by means of a short-time directed transfer function (SDTF) based on a multivariate autoregressive model. The signals from the right (or left) hemisphere were processed simultaneously (not pairwise), which is crucial for obtaining a correct picture of EEG activity transmissions. The pattern of propagation in the beta band involved for both tasks a decrease of the propagation from the motor areas during the execution of the movement ? less pronounced in the case of imagination. The performance of the motion was mainly connected with a short outburst of gamma activity from the hand sensorimotor areas. In case of imagination the gamma outflow lasted longer and concerned larger brain areas.
3
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Molecular biology of prions

75%
Weissmann C., Enari M., Klohn P.-C., Rossi D., Flechsig E.
Acta Neurobiologiae Experimentalis
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2002
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vol. 62
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issue 3
153-166
EN
The ?protein only? hypothesis holds that the infectious agent causing transmissible spongiform encephalopathies is a conformational isomer of PrP, a host protein predominantly expressed in brain and is strongly supported by many lines of evidence. Prion diseases are so far unique among conformational diseases in that they are transmissible, not only experimentally but also by natural routes, mainly by ingestion. The pathway of prions to the brain has been elucidated in outline. A striking feature of prions is their extraordinary resistance to conventional sterilisation procedures, and their capacity to bind to surfaces of metal and plastic without losing infectivity. This property, first observed in a clinical setting, is now being investigated in experimental settings, both in animals and in cell culture.
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