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1

Lactoferrin regulates proliferative response of human peripheral blood mononuclear cells to phytohemagglutinin and mixed lymphocyte reaction

100%
Zimecki M., Stepniak D., Szynol A., Kruzel M. L.
Archivum Immunologiae et Therapiae Experimentalis
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2001
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vol. 49
|
issue 2
147-154
EN
The aim of this study was to investigate the effects of bovine lactoferrin on the proliferative response of human blood lymphocytes induced by PHA and alloantigens in two-way mixed lymphocyte culture at a broad range of BLF concentrations (1.5 - 50 mug/ml). We found that the effects of BLF in both experimental models were differential and depended on an individual reactivity of lymphocytes with respect to mitogen or alloantigen and BLF concentration. Generally, lymphocytes from donors reactive to BLF exhibited higher proliferation indices compared to nonreactive individuals. Low BLF doses were regulatory and higher ones mostly inhibitory. MLR was in most cases inhibited by all doses of BLF, some MLR stimulated and other not affected by BLF. We conclude that basis for the differential action of BLF is its ability to sense the activation status of lymphocytes. The resultant effects of BLF are probably mediated by monocytes and cytokines.
2

Villous trophoblast cell turnover in placentas from preterm pregnancy and pregnancy complicated by intrauterine growth restriction (IUGR)

80%
Zadrozna M., Nowak B., Marcinek A., Duc J.
Folia Biologica
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2010
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vol. 58
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issue 1-2
79-83
EN
Disturbed trophoblast turnover, a key process in placental physiology, may lead to a number of pregnancy-associated pathologies. This study examines PCNA expression and describes and quantifies morphological changes during trophoblast turnover in preterm placentas and termplacentas complicated by IUGR. The number of CTF cells increased two-fold in preterm and IUGR placentas. A concurrent and slightly reduced proliferation rate of these cells was also found. The number of STF nuclei of terminal villi was lower by 21%in IUGR and by 18% in pretermplacentas (P>0.05). A statistically significant reduction of the number of syncytial knots by 50% as compared to the control placentas was observed. Correlations between PCNA-reactive CTF nuclei and syncytial knots, PCNA-reactive CTF nuclei and CCO activity, and CCO activity and syncytial knots were found. Moreover, a strong inverse relation was observed between syncytial knots and CTF cells, and CCO activity and CTF cells.
3

Involvement of beta2-microglobulin in CD69 expression on T cells

80%
Paczek L., Czarkowska-Paczek B., Korczak-Kowalska G., Wierzbicki P., Bartlomiejczyk I., Gorski A.
Archivum Immunologiae et Therapiae Experimentalis
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2001
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vol. 49
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issue 3
239-242
EN
Beta2-Microglobulin (beta2M) is the light chain of the class I HLA molecule. The serum level of beta2M is elevated in various diseases including lymphoma, inflammation, viral infections and chronic renal dysfunction. The present study addressed the possible influence of beta2M on T lymphocyte activation in vitro. Peripheral blood mononuclear cells from a group of 17 healthy subjects were examined. Stimulation with OKT3 and fibronectin in combination with 30 mg beta2M/dl resulted in a two-fold increase of cell proliferation. A similar effect was observed when OKT3 and collagen I were applied as well as when OKT3 and collagen IV were used as costimulation to T cells. The CD69 expression, measured by flow cytometry was significantly enhanced above the control level (1.52 ? 1.03% vs 33.21 ? 20.26%, p<<0.01, control group and 30 mg beta2M/dl, respectively). Together, these observations suggest that beta2M may play a role in modulating lymphocyte proliferation, possibly through modification of the CD69 molecule.
4

The influence of peat extract on the seminiferous epithelium of mouse testes

80%
Bartmanska J., Schuetz A.
Folia Biologica
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1992
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vol. 40
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issue 1-2
83-89
EN
Because of the interest in the peat extract as a potential therapeutic agent its effect on the seminiferous epithelium cells was studied. Adult male mice were intraperitoneally injected with peat extract during 34 days. At intervals equal to the duration of the cycle of the seminiferous epithelium (every 8.5 days)., gametogenic cells were quantitatively analysed. It was revealed that the peat extract causes a decrease in the production of the A1 spermatogonia, and as a result a decrease in the intensity of spermatogenesis>. Besides, in some individuals disturbances of meiosis took place, leading to an increased degeneration of pachytene spermatocytes and formation of diploid speramtids.
5

Anchorage-dependent chick embryo fibroblasts synthesise DNA and proliferate when cultured in multilayered sheets suspended among glass fibres

80%
Czyz J., Dobrucki J., Korohoda W.
Folia Biologica
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2001
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vol. 49
|
issue 1-2
1-12
EN
Chick embryo fibroblasts (CEFs) spontaneously form multicellular and multilayered sheets suspended on the network of glass fibres which are stabilized by fibronectin containing protein deposits located at cell-to-cell contacts. The cells situated within the sheets are surrounded by the neighbouring cells and their mechanical equilibrium is stabilised by intercellular ?parabaric? effects. It was found that CEFs in the sheets retain relatively high mitotic activity corresponding to that observed in sparse monolayer cultures. These cells grew up to much higher local density than in confluent and contact-inhibited monolayer cultures and developed an abundance of microfilament bundles that terminated at vinculin-containing protein complexes. The results presented demonstrate that direct contact with solid substratum, cell-to-cell contacts, local cell density, and intercellular exchange of humoral factors are not directly involved in the density-dependent inhibition of growth observed in monolayer cultures. They also support the concepts concerning the role of mechanical equilibrium of cell membrane and sub-membranous cytoskeleton in the regulation of proliferation of non-transformed cells.
6

The role of hematopoietic growth factors in regulation of differentiation, proliferation and function of phagocytic cells

80%
Czygier M., Kucharewicz B., Szmitkowski M.
Postępy Higieny i Medycyny Doświadczalnej
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1995
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vol. 49
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issue 4
563-579
EN
Hematopoietic growth factors belong to the family of glycoproteins responsible for regulaton, differentiation and proliferation of myelopoietic cells.In this paper the role of these factors in regulation of teh function of pathogenic cells (granulocyte, monocyte, macrophage) is discussed on the basis of literature data.
7

Spreading-independent growth of normal fibroblasts in three-dimensional cultures

80%
Czyz J., Rajwa B., Bzowska M., Dobrucki J., Korohoda W.
Folia Biologica
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2004
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vol. 52
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issue 1-2
19-24
EN
Changes of cell shape resulting from cellular flattening on culture substratum have previously been demonstrated to correlate with mitotic activity of normal animal cells in monolayer cultures. Here, we compared the shapes and proliferation of chick embryo fibroblasts cultured either in multicellular, multilayered sheets extended between glass fibres, or in standard monolayers. Fibroblasts in sheets retained the mitotic activity characteristic of that observed in sparse monolayer cultures, i.e. considerably higher that in confluent monolayers. Morphometric analyses revealed, however, that the cells in sheets were considerably less flattened than in monolayer cultures. These observations indicate that the modulation of culture conditions resulting in multidirectional cell stretching leads to the dissociation of flattening and mitotic activity of normal animal cells, so long as an intracellular stress field, generated by contractile cytoskeleton and stabilised by intercellular contacts, is maintained.
8

The effect of thymic hormones/TFX/ on lymphocyte subpopulations and proliferation after maximal physical effort

70%
Tchorzewski H., Zeman K., Lewicki R., Fornalczyk-Wachowska E., Golinska A., Kantorski J., Majewska E., Pokoca L.
|
|
vol. 40
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issue 2
157-162
EN
The studies were performed on healthy well-trained cyclists. Maximal physical exercise was performed on a Monark bicycle ergometer according to individual schemes. Heart rate amounting to about 200 bts/min and oxygen consumption stabilization were considered as criteria for maximal physical exercise. In this study we have investigated the effect of short-term stimulation of conditioned sportsmen with thymic hormones and evaluated T cell subsets, DR antigen and transferrin receptor expression as well as mitogen-induced proliferation of lymphocytes before and after maximal physical effort. The results suggest that intensive physical exercise may be responsible for transient decrease of CD4/CD8 ratio and mitogen responsiveness, and increase of mononuclear cells number bearing HLA DR + and CD71 antigens. These changes were modified by the treatment with thymic hormones.
9
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Interleukin-1beta affects the macrophage recruitment and proliferation in the injured brain of 6-day-old rat

70%
Pawlinski R., Setkowicz Z., Mlodzinska K., Janeczko K.
Acta Neurobiologiae Experimentalis
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1999
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vol. 59
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issue 4
271-278
EN
Six-day-old male rats received a mechanical lesion in the left cerebral hemisphere. Thereafter, a single dose of either 5, 50 or 500 units (U) of recombinant rat interleukin-1beta (IL-1beta) was injected into the lesion cavity. One or 2 days after the injury, the rats were injected with ^3H-thymidine. Brain sections were subjected to BSI-B4 lectin histochemistry and autoradiography to visualise proliferating and non-proliferating macrophages located within the region of injury. A mitogenic effect of IL-1beta on macrophages was observed on day 2 in brains injected with the lowest 5 U dose of cytokine. Following administration of higher 50 U and 500 U doses, infiltration of the injured tissue by macrophages was significantly intensified on day 1. However, on day 2, dose-dependent reductions of the total number of macrophages as well as their proliferative activity were recorded. The findings suggest that the higher the initial quantity of macrophages, the sooner they disappeared from the injury site. It may therefore be hypothesised that IL-1beta-induced increase in macrophage recruitment at the beginning of the inflammatory response speeded the removal of tissue debris and, therefore, accelerated healing of the injured nervous tissue.
10

Comparison of proliferation and motile activity between human keratinocytes isolated from skin and oral muco

70%
Drukala J., Zarzecka J., Gojniczek K., Waligorska A., Zapala J., Korohoda W.
Folia Biologica
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2005
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vol. 53
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issue 1-2
21-28
EN
Objective: Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. For healing of skin wounds the skin keratinocytes can be replaced by oral mucosa epithelial cells grown in vitro. The presented experiments were carried out in order to compare the proliferation, morphology, and migration between human keratinocytes isolated from human skin and oral mucosa. Materials and methods: Human epidermal and oral mucosa keratinocytes from primary culture were used in all experiments. Cell motility and shape were determined using computer-aided methods. Results and conclusions: It was demonstrated that although both cell types exhibit the same typical epithelial morphology, oral mucosa keratinocytes locomote significantly faster than skin keratinocytes. They also differ in proliferation activity. Oral mucosa keratinocytes exhibited faster growth and different actin cytoskeleton organisation than skin keratinocytes under in vitro conditions. Autologous oral mucosa keratinocytes may be expanded in vitro and used for skin wound healing in vivo.
11
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Effect of palmitoylcarnitine on the cellular differentiation, proliferation and protein kinase C activity in neuroblastoma nb-2a

61%
Nalecz K. A., Mroczkowska J. E., Berent U., Nalecz M. J.
Acta Neurobiologiae Experimentalis
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1997
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vol. 57
|
issue 4
263-274
EN
Palmitoylcarnitine is synthesized through the action of palmitoylcarnitine transferase I - an enzyme specifically inhibited by etomoxir. An increase of the intracellular content of palmitoylcarnitine in neuroblastoma NB-2a cells after administration of carnitine was correlated with an inhibition of cell proliferation and a concomitant promotion of differentiation processes. The activity of protein kinase C was measured in vivo, with cells permeabilized through the use of streptolysin O and a peptide substrate. Palmitoylcarnitine inhibited the phorbol ester stimulated reaction of the peptide phosphorylation in a concentration dependent way. The degree of protein kinase C inhibition was correlated with intracellular increase of the palmitoylcarnitine content, pointing to this compound as a natural modulator of protein kinase C activity.
12

A direct comparison of rejection by CD8 and CD4 T cells in a transgenic model of allotransplantation

61%
Porrett P. M., Lee I. M. K., Lian M. M., Wang J., Caton A. J., Deng S., Markmann J. E., Moore D. J.
Archivum Immunologiae et Therapiae Experimentalis
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2008
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vol. 56
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issue 3
192-200
EN
Introduction: The relative contributions of CD4+ and CD8+ T cells to transplant rejection remains unknown. The authors integrated a previous model of CD4-mediated graft rejection with a complementary model of CD8-mediated rejection to directly compare the function of graft-reactive CD4+ and CD8+ lymphocytes in vivo in a model where rejection requires transgenic T cells. These studies allow direct comparison of CD4 and CD8 T cell responses to the same antigen without the confounding effects of T cell depletion or homeostatic proliferation. Materials and Methods: Clone 4 and TS1 mice possess MHC class I- and II-restricted CD8+ and CD4+ T cells, respectively, which express transgenic T cell receptors that recognize the influenza hemagglutinin antigen (HA). We compared the in vivo response of CFSE-labeled, HA-specific transgenic CD8+ and CD4+ T cells after adoptive transfer into syngeneic BALB/c mice grafted with HA-expressing skin. Results: As in the authors' CD4+ model, HA104 skin was consistently rejected by both Clone 4 mice (n=9, MST: 14.2) and by 5x105 Clone 4 lymphocytes transferred to naive BALB/c hosts that do not otherwise reject HA+ grafts. Rejection correlated with extensive proliferation of either graft-reactive T cell subset in the draining lymph nodes, and antigen-specific CD4+ and CD8+ cells acquired effector function and proliferated with similar kinetics.Conclusions: These data extend the authors' unique transgenic transplantation model to the investigation of CD8 T cell function. The initial results confirm fundamental functional similarity between the CD4 and CD8 T cell subsets and provide insight into the considerable redundancy underlying T cell mechanisms mediating allograft rejection.
13

Processes involved in the repair of injured airway epithelia

61%
Tesfaigzi Y.
Archivum Immunologiae et Therapiae Experimentalis
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2003
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vol. 51
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issue 5
283-288
EN
Recent studies have uncovered many aspects of the repair processes that follow airway epithelial injury. Although the repair process has common elements among various epithelia, such as the ones lining the airways, skin, and gut, there are differences based on their diverse functions. Whenever possible, similarities are pointed out that could help researchers further investigate their application to airway epithelia, although it would be beyond the scope of this review to cover the processes that may occur during the repair of all types of epithelia. In general, five major steps are involved in the recovery of airway epithelia from injury: 1) epithelial cells migrate to cover denuded areas within minutes, and certain proteins, such as the trefoil factor family proteins, are crucial to this process; 2) epithelial cells start to proliferate in order to replace injured cells and to differentiate to establish squamous or mucous cell metaplasia; 3) because more epithelial cells are present after proliferation, some of the cells must be discarded to restore the epithelium to the original condition; 4) once the cell numbers have been reduced to those found in unexposed individuals, the normal proportions of cell types are restored; 5) finally, studies from exposures of rats to ozone show that epithelial cells can adapt and develop a memory of the chronic exposure to which they were exposed. This adaptation allows the epithelium to respond quickly, thus minimizing further injury. Although the molecular mechanisms involved in these major steps of the recovery process are largely unknown, disruption of these steps clearly causes the permanent changes observed in diseases such as asthma, chronic bronchitis, and cancer; therefore, extensive research in these areas may provide ideas for novel therapies.
14

Mesenchymal stromal cells: tissue engineers and immune response modulators

61%
van_ d. B. L. C. J., Figdor C. G., Torensma R.
Archivum Immunologiae et Therapiae Experimentalis
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2008
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vol. 56
|
issue 5
325-329
EN
Mesenchymal stromal cells (MSCs) show significant immune-suppressive properties both in vitro and in vivo. Based on their immune-stealth properties, allogeneic MSCs are used to treat several diseases, for example the injection of MSCs in infarcted heart tissue or their use in bone-cartilage regeneration. The most spectacular treatment was recently described. MSCs were able to down-regulate the severity of graft-versus-host disease, leading to an impressive 20 to 50% increase in the two-year survival of bone marrow transplantation patients. Here the current literature is reviewed to elucidate the different mechanisms involved in these two clinical treatment modalities of MSCs.
15

The effect of transformation with iaglu gene on growth and development of trangenic strawberry in in vitro cultures

61%
Wawrzynczak D., Sowik I., Michalczuk L.
Biotechnologia
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2004
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issue 2
46-53
EN
The aim of the presented work was to study the effects of changes of endogenous indole-3-acetic acid (IAA) metabolizm on in vitro shoot proliferation and rhizogenesis of transgenic strawberry shoots carrying maize IAA-glucose synthase gene (iaglu). Four iaglu-transformed strawberry clones and nontransformed 'Kaster' shoots served as a plant material for the study. The analysis of free and conjugated IAA level in leaves of transgenic and control strawberry plants showed that iaglu-containing strawberry clones had significantly higher level of ester conjugated IAA, but the level of free hormone was only slightly decreased or comparable to the control plants. iaglu-transformed clones had significantly higher proliferation rate and formed more roots than the control shoots. One of the iaglu-transformed clones had significantly shorter and other two ? longer roots than the control plantlets.
16
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Isulfhydryl reagents with K+ transport in adrenal chromaffin granules

51%
Szewczyk A., Lobanov N. A., Nowotny M., Nalecz M. J., Berent U., Mroczkowska J. E., Nalecz K. A.
Acta Neurobiologiae Experimentalis
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1997
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vol. 57
|
issue 4
329-332
EN
In the present study the functional role of SH groups in the Ca2+ -independent K+ selective channel activity in the membrane of bovine adrenal gland chromaffin granules has been studied. Ionic channel activity has been estimated using 86Rb+, a K+ analogue, flux measurements. The inhibition of chromaffin granules K+ channel by SH modifying agents, such as N ethylmaleimide, mersalyl and phenylarsenoxide, is described.
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