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EN
Organs transplantation has become a routine treatment method for patients with terminal organs incapacity. Organs preservation solutions are the key elements of transplantation success. The ideal preservation fluid should be characterized as follows: it contains compounds of the ATP regeneration, minimizes creation of free radicals and cell swelling, prevents intracellular oxidosis, secures specific oncotic pressure and longer storage time (2-3 days or more). The standard preservation solutions used in Poland are: ViaSpan (Belzer solution, UW), HTK (Bretschneider solution, Custodiol), EuroCollins solution and more seldom Celsior.
EN
Food processing using high-intensity pulsed electric fields (PEF) is a new technology to inactivate microorganisms and enzymes. There is only a small increase in food temperature during that process. Inactivation is propably caused by changes in the semipermeable barrier of the cell membrane. High-intensity PEF provides an alternative to conventional thermal methods, where the inactivation of microorganisms implies the loss of valuable nutrients and faste quality.
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EN
Tissue culture has been utilized for producing virus free plants for vegetative propagation and genetic engineering. In addition, altered cells lines showing higher productivity of secondary metabolities can be obtained using tissue culture. The wide use of tissue culture requires the development of new preservation techniques for in vitro culture material. One of this method is growth reduction achieved by modifying various parameters such as: temperature, culture medium, gasous environment. However, crypreservation (i. e. storage in liquid nitrogen. -196?C) is the only method available nowadays for long-term conservation. New cryopreservation techniques such as encapsulation-dehydratation, vitrification and desiccation helped expend the list of plant species that can tolerate low temperatures and are characterized by a normal rate of growth. Each step of the cryopreservation procces requires specific conditions.
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