Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 2

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  POLYPLOID
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Biotechnology in haploids and polyploids

100%
Niemirowicz-Szczytt K.
Biotechnologia
|
1996
|
issue 1
52-59
EN
The progress made by new biotechnologies in haploid and polyploid developments is outlined.There are many applications for biotechnology in haploid production via anther or ovule culture or embryo rescue, clonal propagation, doubling, protoplast fusion, mutant induction and transformation.In several species, transformed germplasm derived from somatic fusion or gene transfer is already being used in fields trials.Meiotic mutants that form unreduced gametes have improved results of crosses between species with different levels of ploidy.Genomic maps based on RFLP technique and doubled haploids as well as physical maps (FISH technique) are under construction.New molecular (RFLP, RAPD) and immunological markers are being used for diagnostic assays.The priorities for biotechnology research in plants are emphasised.
2

Transgene expression and gene silencing in cereals

88%
Nadolska-Orczyk A.
Biotechnologia
|
2006
|
issue 4
168-180
EN
Genetic transformation of cereal crops is a powerful research tool for analysis of gene function and varietal improvement. Application of the method is possible when the expression of introduced transgene is on the desired level and stable over several generations. The production of transgenic cereals was mainly performed by microprojectile bombardment. However, some advance was also achieved by application of Agrobacterium-mediated transformation. For rice, which is the cereal model species, this method is routinely used, while for many others, especially polyploids, it has been developed very recently and only in a few laboratories. We still lack the knowledge whether the main features of Agro-mediated transformation (i.e. integration of one or few copies usually not rearranged and well defined transgene cassette) influence the transgene expression in polyploid cereal species. This review discusses known mechanisms possibly involved in transgene silencing, using both transformation methods. Part of the discussion is focused on transgene expression / silencing in relation to large genomes of polyploid cereals. Another application of genetic transformation, based on RNAi technology (RNA interference), is silencing of selected genes. This could be used to study gene function as well as to induce silencing of the native, single or family genes of cereals. Two strategies of silencing are discussed: a strategy of transcriptional gene silencing (TGS) and posttranscriptional gene silencing (PTGS).
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.