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1

Public perception of biotechnology

100%
Twardowski T.
Biotechnologia
|
2002
|
issue 4
121-129
EN
Public perception is one of key factors for the future development of biotechnology. We surveyed Polish society in years 1998-2001. Significant decrease of trust in biotechnology during this period of time has been found. In conclusion, the necessity of permanent education is stressed.
2

Public perception of biotechnology in Poland; Dynamics of changes in 1998-2000

100%
Twardowska-Pozorska A., Grajek W., Twardowski T.
Biotechnologia
|
2001
|
issue 1
87-100
EN
Public perception of biotechnology in Poland in years 1998-2000 was surveyed. In the survey in general the scheme of research of Eurobarometer was followed. Based on the data of last three years the changes of the opinion of Polish society in comparison to the European data were presented. About 10% reduction of the public trust and willingness to accept biotechnology was found in all social groups, except well educated, well finances group, aged 20-30 years. The conclusion is simple only the social, permanent education will improved public perception of modern technology.
3

The experience of the company producing GM food in Poland

80%
Olejniczak K.
Biotechnologia
|
2002
|
issue 4
115-120
EN
Production of GM food is limited by several factors. The most significant are legislation, public perception and profit. Due to negative effect of these factors the Polgrunt decided for ?IP-non GM? production.
4

Ticks and mosquitoes as vectors of Borrelia burgdorferi s. l. in the forested areas of Szczecin

80%
Kosik-Bogacka D., Kuzzna-Grygiel W., Jaborowska M.
Folia Biologica
|
2007
|
vol. 55
|
issue 3-4
143-146
EN
The aim of the study was to determine the infection level of adult forms and larvae of ticks and mosquitoes with Borrelia burgdorferi in the forested areas of Szczecin. A total of 1699 ticks Ixodes ricinus, including 1422 nymphs, 277 adult forms and 2862 mosquito females representing the genera Aedes (89.6%) and Culex (10.4%) were collected between the years 2004 and 2005. A further 3746 larvae and 1596 pupae of Culex pipiens pipiens were colleted from water bodies. Borrelia burgdorferi s. l. was detected in the arthropods by the method of indirect immunofluorescence assay (IFA). A positive immunological reaction was detected in 16.6% of the adult forms and in 16.5% of the nymphs of Ixodes ricinus. Spirochetes were also detected in 1.7% of mosquito females, 3.2% of larvae and in 1.6% of pupae of Culex pipiens pipiens. The results of the present study confirm that contact with ticks constitutes the main risk of contracting Lyme disease, although mosquitoes play a role as vectors as well.
5

CTLA-4 (CD152) gene polymorphism at position 49 in exon 1 in Graves' disease in a Polish population of the Lower Silesian region

80%
Frydecka I., Daroszewski J., Suwalska K., Zoledziowska M., Tutak A., Slowik M., Potoczek S., Dobosz T.
|
|
issue 5
369-374
EN
Graves' disease (GD) is an autoimmune disease believed to be caused by a combination of environmental and genetic factors. The gene encoding cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is one of the candidate genes for conferring susceptibility to thyroid autoimmunity. The aim of the study was to investigate the association between the exon 1 CTLA-4 gene polymorphism A(49)G and susceptibility to GD and Graves' ophthalmopathy (GO) as well as its severity in a Polish population of the Lower Silesia region. We analyzed the A(49)G exon 1 CTLA-4 gene polymorphism in 99 unrelated Polish patients with GD, of whom 50 had clinically evident GO (NOSPECS class III and higher), and 154 matched healthy subjects from the Lower Silesia region. Genomic DNA was isolated from whole frozen blood using the NucleoSpinR Blood kit. A/G transition was genotyped by polymerase chain reaction followed by labeling with the SnaPshot kit of PE Applied Biosystems and detected using an ABI PRISM 310 capillary genetic analyzer. The distribution of CTLA-4 exon 1 A(49)G genotype, allele, and phenotypic frequencies did not differ between patients with GD and healthy subjects. There was a significantly lower frequency of the AA genotype in the group of patients with clinically evident GO than in patients without severe GO (22% vs. 43%; p=0.02, OR=2.6). Our results showed that the AA genotype in patients with GD is associated with a lower risk of GO severity.
6

Changes in the phytoseston of a river-lake system in Drawienski National Park

80%
Goldyn R., Szelag-Wasielewska E.
Oceanological and Hydrobiological Studies
|
2004
|
issue 2
17-28
EN
The phytoseston in the Plociczna River originated mainly from lakes situated in its water course. It was more abundant and diverse in spring than in summer, which was related to differences in water discharge. The phytoseston abundance between the stations at the inlets and outlets of the successive lakes increased 9.7, 1.9 and decreased 1.4 times, respectively. A gradual reduction in the abundance of organisms was also noted at the outlets of successive lakes, which indicated a decrease in the trophic status of the water. The abundance of phytoseston between the outlets and inlets to the subsequent lakes (river stretch) decreased considerably. Disturbances in the river continuum system caused by the lakes situated in the river course resulted in the acceleration of potamoplankton succession.
7

Farmers opinion about GM plants

80%
Lubiatowska-Krysiak E., Twardowski T.
Biotechnologia
|
2008
|
issue 2
131-141
EN
In 2007 Polish farmers produced 320 ha of genetically modified corn. About 85% of Polish farmers want to have freedom of choice and in 2007 over 70% are ready to breed GM corn. It is important to know, that Polish farmers correctly recognize the profits from GM plants. About 90% of farmers expect solid information from the state administration and labeling of GM products.
8

Dispersion of Didymosphaenia geminata in the flowing waters of southern Poland - new sites of species occurrence in the Orawska watershed and the Orawska basin

80%
Noga T.
Oceanological and Hydrobiological Studies
|
2003
|
issue 4
159-170
EN
To date, Didymosphaenia geminata has been recorded in boreal and montane regions in Europe, Asia and North America. It has been recorded only rarely in central Europe in cold, oligotrophic montane water bodies with a moderately low electrolyte content. In Poland, it occurred in the streams in the Tatra Mountains and in the Dunajec River but abundance was low. During the 1990s this species appeared in the Carpathian tributaries of the Vistula River (So?a, Skawa and Raba). Very high Didymosphaenia geminata abundance was noted in the San River below the dams in mezotrophic environments. Didymosphaenia geminata was recorded for the first time in mezotrophic environments in the Czarna Orawa River and its tributaries, most of which - Zubrzyca, Sylec and Lipnica - come from Babia G?ra Mountain. The highest abundance was noted in the Zubrzyca stream where there were macroscopic aggregations. The most common species in these aggregations were Navicula capitatoradiata, N. lanceolata, Cymbella minuta, Achnanthes biasolettiana var. biasolettiana, Fragilaria arcus var. arcus, F. ulna, Diatoma vulgaris and Cocconeis placentula var. euglypta
9

Densities and morphology of two co-existing lizard species (Lacerta agilis and Zootoca vivipara) in an extensivelly used farmland in Poland

80%
Ekner A., Majlath I., Majlathova V., Hromada M., Bona M., Antczak M., Bogaczyk M., Tryjanowski P.
Folia Biologica
|
2008
|
vol. 56
|
issue 3-4
165-171
EN
The study was carried out in extensive farmland area near the town of Odolan?w, Poland. During two breeding seasons (April-May, 2006-2007) lizards were counted on transect routes and captured by hand or by noosing. In total, 123 specimens of L. agilis and 153 specimens of Z. vivipara were captured. The proportion of males to females wasn.t differed from the theoretical 1:1 ratio. Almost half of the individuals exhibited tail autotomy at least once in life. In the studied sand lizards significant sex specific differences were found between all morphological traits, i.e. males were shorter, lighter, but had a bigger head. In common lizards significant sex specific differences were detected only in body length, i.e. females were longer. All of the morphological traits were highly intercorrelated.
10

Trends in diagnosis and prevalence of critical congenital heart defects in the Podkarpacie province in 2002?2004, based on data from the Polish Registry of Congenital Malformations

80%
Gorska-Kot A., Blaz W., Pszeniczna E., Rusin J., Materna-Kiryluk A., Homa E., Hejda G., Franus J.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 2
191-194
EN
This paper presents results of data collection regarding congenital heart defects (CHD) for the Polish Registry of Congenital Malformations (PRCM) from the Podkarpacie province in 2002?2004. Routine methods (fetal echocardiography, clinical examination) and recently also unique methods (screening echocardiography and pulse oximetry) were used for early detection of critical cardiac malformations. Critical CHD were detected there in 107 newborns in 2002?2004, so the mean prevalence reached 1.71/1000. Within this group, death rate decreased from 8 deaths in 2002 to 2 deaths in 2004, mainly thanks to improved detection and treatment of CHD.
11

The accessibility of coastal environmental information through the Internet (Mermaid - the Internet Data Broker concept)

80%
Krzyminski W., Kaminska K. M., Kaminska M., Kozakiewicz K. A., Kozakiewicz A., Naguszewski N. A., Naguszewski A., Franz F. M., Franz M.
|
EN
The paper gives an overview of the modern concept of Internet data broker for environmental data transfer and exchange thorough the Internet. The main tasks of the specific data broker components are to catalogue data (metadata database), to search for data, to retrieve data subsets, to package data, to dispatch data and finally to charge for the data. These issues are covered by the activities within the EU 5th Framework Programme known as Marine Environmental Response data Management and Acquisition using Internet Data brokerage, or MERMAID. The main idea of the project is that many users are linked through the Internet Data Broker with various environmental datasets made available by data providers.
12

Cytotaxonomic characteristics of the genus Glyptotendipes Kieffer (Chironomidae, Diptera) from fish and retention ponds (Silesia, southern Poland)

80%
Michailova P., Warchalowska-Sliwa E., Kownacki A.
Folia Biologica
|
2001
|
vol. 49
|
issue 3-4
163-173
EN
The cytotaxonomic characteristics of species of the genus Glyptotendipes (Chironomidae): G. glaucus Meigen, G. paripes Edwards, and G. barbipes (Staeger) are described. The studied material was collected from a fish pond at Golysz and a sewage retention pond at Chybie in Silesia. All the studied species have the chromosome set 2n=8, but with many specific structural and functional changes. The cytogenetic data of G. glaucus showed that the studied specimens of this species have been produced by the introgressive hybridization of two sibling species: G. glaucus x G. pallens, and subsequent crossing-over in the hybrid chromosome CD. Owing to this process, the band pattern of chromosome arm D coincided with those of G. pallens. New aberrations (homo- and heterozygous deletions in arm G as well as heterozygous inversions in chromosome arm B) were detected in G. glaucus. Specific band sequences were discovered in chromosomes of G. paripes. The band patterns established in chromosomes AB and G of this species were identical with those of Siberian populations. The banding patterns of the polytene chromosomes of G. barbipes do not differ from the standard. However, high frequency of pericentric inversion of chromosome AB was established. Many new puffs were found in the polytene chromosomes of all the studied species. Their nucleolar organizer was very sensitive to environmental changes. In G. glaucus it appeared in three different states: very active, slightly active, and heterozygous state. The organic pollution existing in the sewage retention pond may contribute to possible mutations and chromosomal damage in Glyptotendipes species. Structural and functional rearrangements of the polytene chromosomes of these species mobilized their genomes and provide for survival under polluted conditions.
13

The distribution of water vegetation on the Polish coast of the Baltic Sea in 1996-2000

80%
Plinski M., Jozwiak T.
Oceanological and Hydrobiological Studies
|
2004
|
issue 2
28-40
EN
Qualitative analysis of vegetation along the Polish coast was conducted. Thirty-six species were noted - 16 of green, 5 of brown and 7 of red algae and 8 of vascular plants. In the 1996-2000 period, the share of green algae was always more than 50% of the floral composition. Only a few species were present throughout the period of investigations, as follows: green algae - Enteromorpha ahlneriana, E. intestinalis, Cladophora albida, Cl. glomerata, Cl. rupestris, Cl. sericea, Cl. vagabunda; brown algae - Pilayella litoralis, Fucus vesiculosus; red algae - Polysiphonia violacea, Ceramium diaphanum; vascular plants - Zostera marina. Some general conclusions can be drawn with reference to the geographical distribution of the species. Enteromorpha intestinalis was the only species that occurred at every station in almost every year. Three species of the genus Cladophora (Cladophora albida, Cl. rupestris, Cl. sericea) and Pilayella litoralis were also very common. Zostera marina was nearly absent from the central part of the Polish coast. Other species occurred rarely and accidentally.
14

What Polish people know about modern biotechnology

80%
Twardowska-Pozorska A., Grajek W., Twardowski T.
Biotechnologia
|
2001
|
issue 4
115-121
EN
Continuing our research on public perception of modern biotechnology in Poland this time we surveyed the knowledge of modern biotechnology between Poles. The representative sample of Polish society (over 1000 Poles aged over 15 years) answered questions like: did you eat genes for breakfast?, are genes in tomato?, is BSE a product of genetic engineering? We found surprisingly good familiarity (awareness) of background biotechnology. Over 40% of Poles know that all tomatoes contain genes, over 20% eat genes for breakfast and one third know that BSE is not a result of genetic engineering.
15

Lack of association between Exon 1 CTLA-4 gene polymorphism A(49)G and multiple sclerosis in Polish population of the Lower Silesia Region

80%
Bocko D., Bilinska M., Dobosz T., Zoledziewska M., Suwalska K., Tutak A., Gruszka E., Frydecka I.
Archivum Immunologiae et Therapiae Experimentalis
|
2003
|
vol. 51
|
issue 3
201-205
EN
Multiple sclerosis (MS) a chronic inflammatory demyelinating disease of the central nervous system (CNS) is believed to have a T-cell mediated autoimmune etiology. The cytotoxic T lymphocyte antigen 4 (CTLA-4) gene is a strong candidate for the involvement in autoimmune diseases because CTLA-4 plays an important role in downregulation of early and late stages of T cell activation and maintenance of peripheral T cell tolerance. To examine the genetic association of the CTLA-4 gene locus with MS, we analyzed exon 1 CTLA-4 gen polymorphism A(49)G in 102 unrelated Polish MS patients in Lower Silesia region and 101 age and sex matched healthy subjects. The distribution of CTLA-4 exon 1 A(49)G genotype, phenotype and allele frequencies did not differ between patients with MS and healthy subjects.
16

Applicability of diatom indices for monitoring water quality in coastal streams in the gulf of Gda?sk region, northern Poland

71%
Zgrundo A., Bogaczewicz-Adamczak B.
Oceanological and Hydrobiological Studies
|
2004
|
issue 3
31-46
EN
This aim of this study was to test the applicability of diatom indices developed in Europe for monitoring coastal stream waters in northern Poland. Water, sediment, and diatom samples were taken from streams in the resort town of Sopot that discharge into the Gulf of Gdansk. The physical and chemical measurements taken during the sampling period indicated that the purity of the stream waters had improved, and recently they were classified as I class water purity. This is contrasted by the studied waters? diatom communities, which were dominated by eutraphentic taxa. High abundances of -mesosaprobic and -mesosaprobic taxa were recorded at most of the sites. Diatom indices developed for water quality surveillance were calculated with the Omnidia3 database software. Redundancy analyses (RDA), an ordination technique, was used to determine the indices that best correspond to water pollution in coastal streams. The results of the present study indicate that the pollution indices that follow can be applied successfully to monitor flowing freshwaters in the Gulf of Gdansk area in northern Poland: IDAP (Indice Diatomique Artois-Picardie); SPI (Specific Polluosensivity Index); EPI-D (Eutrophication/Pollution Index based on Diatoms), which integrates organic pollution, eutrophication, and mineralization.
17

Seasonal occurrence of parasites of flounder Platichthys flesus (L.) from the Gulf of Gdansk

71%
Chibani M., Rokicki J.
Oceanological and Hydrobiological Studies
|
2004
|
issue 3
17-30
EN
A total of 1509 specimens of flounder Platichthys flesus (L.) were sampled in the period from April 1998 to March 2000 from the Gulf of Gdansk. The seasonal occurrence of all parasite species was calculated. The results indicated that some parasite species occur seasonally (Diplostomum sp., Raphidascaris acus, Dichelyne minutus, Echinorhynchus gadi and Pomphorhynchus laevis), while others do not (Trichodina borealis, Hysterothylacium aduncum, Cucullanus heterochrous). Since some parasites occurred year-round at low levels of infection, it was difficult to determine if they exhibited seasonality or not (Glugea anomala, Gyrodactylus unicopula, Diplostomum mergi, Bothriocephalus scorpii and Lampetra sp.). Gyrodactylus flesi was noted occasionally
18

Preliminary Investigations of psammon rotifers in two reservoirs in Upper Silesia

71%
Bielanska-Grajner I.
Oceanological and Hydrobiological Studies
|
2004
|
issue 1
37-45
EN
Psammon rotifers were investigated in two lakes of different trophy and degrees of pollution. The Plawniowice Duze Reservoir is mesotrophic, while the Dzierzno Duze Reservoir is highly eutrophic and polluted. Samples were collected from three zones on sandy beaches - one meter above the shoreline (eupsammon), at the shoreline (hygropsammon) and below the water level (hydropsammon). A larger number of rotifer species (28) was detected in the Plawniowice Duze Reservoir than in the Dzierzno Duze Reservoir (18). The species structure of the rotifer communities differed in the two reservoirs. While the density of eupsammic rotifers observed in both reservoirs was high, significant differences were noted in the abundance of them. The dominant species were Cephalodella gibba, Cephalodella catellina and Lecane closterocerca.
19

Interdependence between phosphorus forms in sediments and iron in interstitial waters in the Gulf of Gdansk

71%
Matuszewska K., Bialkowska I., Bolalek J.
Oceanological and Hydrobiological Studies
|
2003
|
issue 1
5-14
EN
The content of various phosphorus forms in sediments and the content of iron in interstitial waters were measured in sediment samples collected in the Gulf of Gdansk in March 2001. The studies showed that the greatest amounts of the total phosphorus and total dissolved iron were present in the uppermost sediment layer, and their respective concentrations ranged from 203,99 mumol?g-1d.w. to 1894,02 mumol?g-1d.w., and from 0,02 mumol?dm-3 to 4,68 mumol?dm-3. The contents of these parameters were directly connected with the type of sediment - the greatest concentrations were measured in fine sediments. The analysis of multiple correlation coefficients demonstrated that in over 90% of cases the concentration of phosphorus bound with iron depended on: the sediment type and its humidity, the content of organic matter, and the concentration of the total iron dissolved in the interstitial waters.
20

Restriction fragment length polymorphism of exon 2 Ovar-DRB1 gene in Polish Heath Sheep and Polish Lowland Sheep

71%
Gruszczynska J., Brokowska K., Charon K. M., Swiderek W. P.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 3
311-314
EN
Exon 2 of the Ovar-DR gene is known to encode the MHC outer domain (alpha or beta chain) that forms the binding area to antigens presented. The study was aimed at analysing exon 2 Ovar -DRB1 gene polymorphism in Polish Heath Sheep and Polish Lowland Sheep (Zelazna variety). A total of 101 and 99 ewes of the respective breeds were included in this study. We identified 65 different haplotypes in Polish Heath Sheep and 68 in Polish Lowland Sheep. The PCR-RFLP method and PCR products sequencing made it possible to identify two new sequences of exon 2 Ovar-DRB1 gene (AY230000 and AY248695). A distinct polymorphism in the exon 2 sequence presents possibilities for immune response toward a great variety of pathogens.
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