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1

Early stages of embryogenesis after fertilization in vitro condition

100%
Dziedzic E., Lech W., Malodobry M.
Biotechnologia
|
2001
|
issue 2
54-56
EN
Ovules of plum cultivars (Sweet Common Prune, Herman, Gilbert, Sanctus Hubertus, Cacanska Rodna, Cacanska Najbolia) were pollinated with pollen of cv. Stanley on the White?a medium with addition of 15% sucrose. The nucelluses excised from the fertilized ovules were cultivated on Norstog medium with addition of 3,4% sucrose. The process of fertilization after pollination in the in vitro condition was proved on the basis of the paraffin cross?sections: the degeneration of synergid after entering the pollen tube, the fertilization of egg cell were confirmed. The microscopic observations showed the swelling of micropylar part of nucellus. The paraffin cross-sections showed a many-layer kalotka (peculiar morphological structure) and swelled cells of nucellus among which the embryo sac was observed. The presence of embryo without endosperm or the presence of endosperm without embryo in the embryo sac was noted.
2

Analysis of plum (Prunus domestica L.) DNA polymorphism using RAPD - PCR technique

100%
Lisek A., Korbin M., Zurawicz E., Rozpara E.
Biotechnologia
|
2004
|
issue 2
76-72
EN
Precise identification of plum cultivars is desired in breeding programme of the species as well as in orchard practise. The aim of the presented studies was the determination of 19 plum cultivars Prunus domestica L. diversity. RAPD-PCR technique with 77 primers (Operon Technologies) from kits OPB, OPG, OPT and OPU was used for the analysis. Fifty-five polymorphic fragments DNA (600-2700 bp) were obtained in reactions with 33 primers. The highest number of polymorphic fragments (3-5) was observed in reactions with primers OPB 07, OPB 18, OPG 09, OPG 10 and OPT 14. The reactions with OPB 07, OPB 18 and OPG 09 allowed to diversify 15 cultivars, except 'Wegierka Zwykla' and clones 'Promis', 'Tolar', 'Nectavit'.
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