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Plastids in callus tissue culture of Plantago asiatica L.

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Makowczynska J., Andrzejewska-Golec E., Marek K.
Biotechnologia
|
2004
|
issue 2
275-281
EN
For the last few years, in the Department of Biology and Pharmaceutical Botany ? Plantago asiatica L. ? a medicinal plant, popular in the Far East, has been cultured in vitro. Two lines of the nonmorphogenic callus were obtained: cream- and green- coloured. They were induced from the roots taken from 4-week-old seedlings. The cream-coloured callus was cultured on Murashige and Skoog medium enriched with 0.5 mg/dm3 2,4- D and 0.2 mg/dm3 BAP. The green callus was initiated on the same medium supplemented with 0.1 mg/dm3 IAA and 2 mg/dm3 BAP and next it was cultured on MS with 0.5 mg/dm3 IAA and 0.2 mg/dm3 BAP. The fragments of the callus were studied using the light microscope and the transmission electron microscope. For cream-coloured callus, proplastids and amyloplasts were observed. In green callus, in addition to the plastids found in cream-coloured callus, also chloroplasts were observed.
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