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Plant viruses as vectors for foreign gene expression in plants

100%

Palucha A.

Biotechnologia

2002

issue 1

105-112

Several studies have demonstrated the use of plant viruses as vehicles to introduce and express nonviral genes in plants. Many plant viruses multiply to intensely in plants, leading to concomitantly high levels of nonviral gene expression. Plant virus expression vector technology can be improved along several lines. Not all viruses are equally sensitive to sequence manipulations and may be less prone to insert instability. New vectors and new expression strategies are needed to expand the range of plants in which the systems can be used. To increase the carrying capacity or the ability to express more than one nonviral gene, new vectors should be developed.

Infectious cDNA clones of plant viruses

80%

Sadowy E., Zagorski W., Hulanicka D.

Biotechnologia

1996

issue 3

60-68

Obtaining of infectious clones corresponding to the genomes of RNA viruses has greatly enhanced investigations.The different strategies employed to construct infectious clones from plant RNA virus and parameters affecting infectivity of such clones have been described.The system represent a powerful tool fo studying plant virus replication, short and long distance virus movement and the virus - host interactions.It may serve as autonomously replicating vectors for the expression of foreign genes in plants.

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2 Biotechnologia

1 2002

1 1996

1 Hulanicka D.

1 Palucha A.

1 Sadowy E.

1 Zagorski W.

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Plant viruses as vectors for foreign gene expression in plants

Infectious cDNA clones of plant viruses