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The review article presents data on the ethylene emanation by bacteria, the two different pathways of its biosynthesis in these microorganisms and the role of ethylene in plant pathogenesis. ACC deaminase from Pseudomonas and Enterobacter spp., which catalyses the hydrolytic cleavage of ACC in higher plants was also discussed.
EN
Systems are required which allow selection of transformed cells. Genes that confer a selectable advantage to transformed cells are included in the transformation system. They include the use of antibiotics, herbicides or other compounds. In this review, we describe the systems developed for selective elimination of selectable marker genes from the transgenic plants.
EN
A great progress in the higher plants transformation methodology has been observed. It includes the following events: increasing efficiency, development of new markers which are ecologically acceptable, transformation of new monocotyledonous species using A. tumefaciens 'supervectors', control of recombination site.
EN
Possible interactions between proteins and nucleic acids which might contribute to general effectivity op plant genetic transformation systems are reviwed. Experimental data concerning direct and A tumefaciens - based plant genetic transformation systems are compared with data obtained from research on nucleocytoplasmic exchange machanisms of biopolmers.In thaht context, mechanisms of hnRNA interactions with hnRNP appear to be of great importance. This type of interactions is determined by conservative nucleic acida bindng domains of many cellular proteins e.g. RGG and RBD.These proteins seem to play an important role in the integration of many other cellular processes.
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