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1

Substrate specificity and sensitiveness of phenol monooxygenase from Stenotrophomonas maltophilia strain KB2 versus their potential application to bioremediation of the environment

100%
Wojcieszynska D., Gren I., Labuzek S., Respondek M.
|
2007
|
issue 2
181-191
EN
Phenol monooxygenase, isolated from Stenotrophomonas maltophilia strain KB2, was sensitive to sodium azide, metals salts except for iron (II) sulfate at concentration of 1 mM, chelate compounds, sulfhydryl agents, lauroylsarcosine Na-salt, SDS and hydrogen peroxide. Slight increase of the enzyme activity was observed in the presence of hexane and ether. The presence of ascorbic acid caused an increase of the enzyme activity. Phenol monooxygenase activity changed significantly depending on the tested aromatic substrate in the reaction mixture and the type of the applied inductor.
2

Immobilized enzymes in the biodegradation process of phenols and cyanides contained in industrial waste water

88%
Bodzek M., Bohdziewicz J., Kowalska M.
Biotechnologia
|
1993
|
issue 2
121-133
EN
The paper deals with removing phenols and cyanides from industrial waste waters, as a result of their biological degradation by means of ultrafiltration membranes with immobilized enzymes coming from bacterial strain Pseudomonas sp. The enzymatic membranes were obtained by ultrafiltration of protein solution trough a membrane made of polyacrylonitrile. Is this way,above 90 percent of the enzymes were adsorbed onto the membrane surface. Such enzymatic membranes were decomposed phenol and potassium cyanide in 70 and 80 percent respectively, during single ultrafiltration at the pressure of 100000 Pa and at two fold reduction of initial volume of waste waters. The retention coefficient of ultrafiltration process amounted to 100 percent for phenol and was above 90 percent for potassium cyanide.
3

Changes in cellular fatty acids composition induced by phenol and catechol in Pseudomonas vesicularis and Pseudomonas stutzeri

88%
Mrozik A., Labuzek S., Piotrowska-Seget Z.
Biotechnologia
|
2004
|
issue 1
244-252
EN
The changes in cellular fatty acid profiles, determined by gas chromatography, of Pseudomonas vesicularis and Pseudomonas stutzeri growing in a modified minimal medium with catechol or phenol are presented in this paper. P. vesicularis increased its ratio of saturated/unsaturated fatty acids from 1.92 to 4.05 and 5.72 when grown on glucose, phenol and catechol, respectively. In the case of P. stutzeri, the ratio changed from 2.56 to 8.28 and 4.65 under the same growth conditions. The increase in the abundance of saturated and cyclopropane fatty acids and forming new iso and anteiso fatty acids during growth of tested strains in the presence of catechol and phenol are suggested as a possible mechanism to tolerate tested aromatic compounds.
4

Influence of the phenol substances on shoot buds development of apple rootstock M7 in tissue culture

88%
Plazuk E., Kromer K.
Biotechnologia
|
2004
|
issue 2
146-154
EN
The in vitro culture of the apple-tree rootstock M7 was established from apical and lateral shoot meristems as initial explants. The growth of lateral buds, which leads to branch development is limited by the apical dominance. The effect of exogenous growth regulators on axillary bud stimulation was examined. Additionally, the relationship between the position of axillary buds on the stem and their potential growth were investigated. The results indicate that buds from the upper region, near the shoot apex, developed better in comparison with those located below. The participation of endogenous inhibitor of gradient distribution in stem is suggested. We analysed the possible role of phenolic compounds on the initiation and growth stimulation of axillary buds. Several phenols which are known to be specific for apple rootstock M7 was examined to evaluate their effect on bud development.
5

High-quality plant DNA extraction for PCR: an easy approach

63%
Ahmed I., Islam M., Arshad W., Mannan A., Ahmad W., Mirza B.
Journal of Applied Genetics
|
2009
|
vol. 50
|
issue 2
105-107
EN
Polymerase chain reaction has found wide applications in modern research involving transformations and other genomic studies. For reproducible PCR results, however, the quantity and quality of template DNA is of considerable importance. A simple and efficient plant DNA extraction procedure for isolation of high-quality DNA from plant tissues is presented here. It requires maceration of plant tissue of about 1.0/ cm2 (e.g. of a leaf blade) in DNA extraction buffer (100 mM Tris-HCl, 100 mM EDTA, 250 mM NaCl) using 1.5-mL microfuge tubes, followed by cell lysis with 20% SDS, and DNA extraction with phenol: chloroform: iso-amyl alcohol (25:24:1). Hydrated ether is then used to remove polysaccharides and other contaminants from the DNA preparation. Average DNA yield is 20-30 mug/ cm2 for fresh tissues, and ratio of absorbance at 260 nm to absorbance at 280 nm is 1.5-1.8. The DNA is quite suitable for PCR using microsatellites, RAPD and specific markers for recombinant selection. Amplifications have been obtained for these markers by using template DNA extracted from fresh as well as frozen leaf tissues of various plants, including barley, oat, potato and tomato. DNA stored for more than 2 years has been successfully amplified with microsatellite markers, which shows suitability of this method after long-term storage of DNA. Besides, the ease of use and cost-effectiveness make the procedure attractive.
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