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Myosin VI is associated with secretory granules and is present in the nucleus in adrenal medulla chromaffin cells

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Majewski Ł., Sobczak M., Rędowicz M.
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2010
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vol. 57
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issue 1
109-114
EN
Myosin VI (MVI) is the only known myosin walking towards minus end of actin filaments. Here, MVI, but not myosins IB or IIB, was detected in chromaffin granules isolated from bovine medulla and found to be tightly associated with the granule apical surface. MVI also localized to secretory granules within rat pheochromocytoma PC12 cells as well as to the Golgi apparatus, endoplasmic reticulum and clathrin-coated pits. Notably, it was also found in the nucleus. RT-PCR revealed that MVI splice variants with a large insert (LI), characteristic of polarized cells, were barely detectable in PC12 cells, whereas variants with a small insert (SI) were the major isoforms. The presented data indicate that MVI in adrenal medulla cells is engaged in secretory vesicle trafficking within the cytoplasm and possibly also involved in transport within the nucleus.
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Phyllodulcin Protects PC12 Cells Against the Injury Induced by Oxygen and Glucose Deprivation-Restoration

88%
Hu C.-L., Ge L., Tang Y., Li J., Wu C.-H., Hu J.-H., Yuan J.-T., Fan Y.-Z.
Acta Poloniae Pharmaceutica - Drug Research
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2019
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vol. 76
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issue 6
1043-1050
EN
Phyllodulcin is a natural coumarin derivative found in Hydrangea macrophylla. To discover effective therapeutics for cerebral ischemia-reperfusion injury, we investigated the protective effects of phyllodulcin on PC12 cells injury induced by oxygenation and glucose deprivation/restoration (OGD/R). As a result, phyllodulcin can improve the cell viability and attenuate the extracellular lactate dehydrogenase release. Meanwhile, phyllodulcin can ameliorate the dysfunction of mitochondria through reducing reactive oxygen species production, decreasing the intracellular calcium level and increasing the mitochondrial membrane potential. ROS-associated OGD/R can induce cell apoptosis through the mitochondrial pathway. Phyllodulcin can inhibit apoptosis of PC12 cells through down-regulation of Caspase-3 and Bax as well as up-regulation of Bcl-2. These results indicate phyllodulcin can protect PC12 cells against the damage induced by OGD/R. This investigation gives promising evidences for the therapy of cerebral ischemia-reperfusion injury.
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