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  1. 1 Biotechnologia

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  1. 1 1997

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  1. 1 Lamparska K.

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Is parallel, intramolecular association of 5'-->3' oligoribonucleotide strand possible in RNA under physiological conditions ?

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Lamparska K.
Biotechnologia
|
1997
|
issue 2
65-70
EN
The article briefly presents the design and chemical synthesis of parallel stranded DNA with formation of reverse Watson-Crick base pairs. Tchurikov et al attempted an analysis of parallel RNA-RNA duplex formation. Single stranded RNA, prepared by in vitro transcription with T7 polymerase using 33P and 3H nucleoside 5'-triphosphates, were appropriately hybridized and analysed by polyacrylamide gel electrophoresis prior and after RNAze A digestion. Results obtained allow to conclude on the formation of parallel stranded, RNaze A - stable RNA. The presented data show the importance of intramolecular parallel association of the single stranded RNA during its folding.
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