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  1. 1 Biotechnologia

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  1. 1 2004

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  1. 1 Dobras M.

  2. 1 Urbanek H.

  3. 1 Wielanek M.

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Regeneration of Oxalis triangularis from hairy roots and the influence of glutathione on anthocyanin production

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Wielanek M., Urbanek H., Dobras M.
Biotechnologia
|
2004
|
issue 2
260-266
EN
The Oxalis triangularis transformed plants were regenerated from hairy roots induced by A. rhizogenes LBA 9402. In vitro shoot proliferation of the transformed roots-regenerated plants was 65% higher by than that of the non-transformed ones. In vivo the hairy root syndrome was observed for the transformed roots-regenerated plants. Anthocyanin content in the leaves of non-transformed plants was about 40% higher than in the transformed roots-regenerated ones. In the non-transformed plants, GSH (1,5 mM) enhanced anthocyanin production by 30% at the most, while in the transformed roots-regenerated plants the maximal increase in its production was found on the 2nd and 9th days after the treatment, it reached 230-320% and 750-600% of control, respectively.
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