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EN
A method of grasshopper embryo karyology was adopted for Tettigoniidae. Additionally, detailed information of the C-banding pattern of embryos of Tettigonia cantans from the Novosibirsk population is given.
EN
Karyotypes and C-heterochromatin distribution of Deracanthina deracanthoides, Deracanthella verrucosa and Zichya baranovi were studied. Differences in the chromosome numbers, their morphology, and the position of C-bands between species of Bradyporinae are discussed.
EN
Karyotypes, C-bands distribution of nine species belonging to 6 genera, and chromosomal location of NORs in some species were studied. Differences in the chromosome numbers, their morphology, and the position of C-bands between species of Poecilimon and IIsophya are discussed.
EN
The randomamplified polymorphicDNA(RAPD-PCR)methodwas used to study the genetic polymorphism of 20 species of the genus Isophya. Each primer amplified a different set of DNA fragments, all oligonucleotides failed to generate any specific diagnostic band that could lead to the identification of Isophya species, and none of the amplified fragments were present in all species. RAPD markers detected a high level of polymorphism in all species. The data were in most cases not congruent with morphological subdivision to the species group and cytotaxonomic studies. The genetic lin with relationships proposed by systematists.
EN
The karyotypes of species belonging to the Tetrigidae is characterised by structural conservatism. The standard chromosome set of T. japonica, T simulans, T. bolivari, P. meridionalis, U. depressus, and F. robustus consists of 2n=13 acrocentric chromosomes in males and 2n=14 in females, with a sex determining mechanism of X0 male and XX female. C-bands distribution of ten species belonging to 4 genera were studied. Differences in the position of C-bands and number of chiasma between species are discussed.
EN
Karyotypes and C-banding patterns of four species belonging to three genera of the subfamily Phaneropterinae were studied. The basic karyotype of Isophya kalishevskii, Polysarcus zacharovi, and Poecilimon ukrainicus consists of 2n=31(XO) in the male. The chromosome number of Isophya hemiptera is 2n%=28+neo-X+neo-Y as a result of mutual tandem translocation between the originally acrocentric X-chromosome and acrocentric medium size autosome. Analysis of the meiotic behaviour of the neo-X and neo-Y demonstrated a post-reductional division of these chromosomes.
EN
The karyotype of Apteranabropsis tonkinensis, described for the first time, is characterized by 2n_= 19 (XO) and 2n_=20 (XX). Successive stage of spermatogenesis were analysed.
EN
The grasshopper Podisma sapporensis consists of two main chromosome races in Hokkaido. The western group of populations of P. sapporensis, belonging to the XO race, has a diploid number of chromosomes 2n=23 in the male and 2n=24 in the female (sex determination XO male/XX female). The eastern group of populations of this species, belonging to the XY race, differs from the western one as a result of Robertsonian translocation between the originally acrocentric X chromosome and M5 autosome in homozygous state, having resulted in the forming of chromosome sex determination neo-XY male/neo-XX female (2n=22). These races are geographically isolated by the mountainous system consisting of the Mts Daisetsu and Hidaka range, occupying the central part of the island. The hybrid zones between the races have not so far been discovered. Various levels of polymorphism for the pericentric inversions and C-banding variation exist in different chromosomes throughout populations in both chromosome races. In some solitary populations (the population at the summit of Mt Yotei, populations in the vicinity of Naganuma, Oketo, and Tanno) pericentric inversions are fixed in some pairs of chromosomes, which enables marking of the discrete karyomorphes. In the Mt Daisengen population all chromosomes are two-armed as a result of fixing the pericentric inversions. These facts contradict karyotypical conservatism of the tribe Podismini. The level of diversity of P. sapporensis karyotypes could provide a new perspective on the evolutionary process of different karyotype in Orthoptera. The considerable occurrence of polymorphism in chromosomes suggests that karyotypic diversification is undergoing in P. sapporensis. The authors also proposed that P. sapporensis would be divided into four chromosome subraces in the XO chromosome race and two chromosome subraces in the XY race, on the basis of karyotypic features. These races may have been established by fundamental climatic changes during the glacial epoch.
EN
Chromosome numbers and C-banding patterns in the spermatogenesis of seven species of Pamphagidae grasshoppers from the Caucasus, Central Asia, and Trabsbaikalia region of Russia are reported. The patterns of origin and evolution of the neo-XY/neo-XX sex determination in Pamphagidae are discussed.
EN
Tettigonia ussuriana and T. dolichopoda maritima differ in the length of tegmina, details in venation, and in females in details of the subgenital plate. The two species of the genus Tettigonia have the same number and morphology of autosomes but a different morphology of the X chromosome: in T. ussuriana it is metacentric, whereas in T. dolichopoda maritima acrocentric. In both species, euchromatic zones and breaks of one or to chromatids during meiosis and mitosis in theXchromosome were observed. Additionally, B chromosomes were noted in most individuals of both species.
EN
The C-banding patterns in the embryo chromosomes of the grasshopper Podisma pedestris (L.) from the Altai Mts are reported. The additional second C-heterochromatic arms in at least five pairs of autosomes and in the X-chromosome were revealed. The paracentromeric, interstitial, and telomeric C-bands were observed. The studied population of P. pedestris shows some differences in the distribution and amount of the heterochromatin in comparison with European populations.
EN
So far, only about 400 species, subspecies, and chromosome races of 15 subfamilies of Tettigoniidae have been studied karyologically, this constituting about 7% of all described species in this group. An attempt was made to establish the basic diploid chromosome numbers of Tettigoniidae and, considering chromosome number, morphology, and the sex determining mechanisms, to suggest how karyotype evolution in the particular subfamilies could have occurred.
EN
Cytotaxonomic analysis of 20 species and subspecies of the genus Poecilimon using C-banding pattern, chiasma frequency, and morphometric characteristics of the chromosomes were described. Using a cladistic analysis the chromosome data provided a basis to produce a phylogenetic tree which was compared with a tree based on morphological characters and DNA sequence data. There are importent differences in the grouping of data sets to species obtained on the basis of morphology/DNA analyses and that based on chromosomes. The explanation of the differences between C-banding patterns and taxonomic proximity is probably that the C-banding pattern changes quickly as the result of the high degree of variation of constitutive heterochromatin.
EN
The results of comparative karyotypical studies of the Caelifera orthopteran insects from Russia, Kazakhstan, Central, Asia and the Caucasus are summarized. Chromosome complements for 166 species belonging to the families: Eumastacidae (subfamily Gomphomastacinae; 2n male=19,21), Tetrigidae (2n male=13), Acrididae (2n male=23, 22, 21, 19, 17, 16), Pyrgomorphidae (2n male=19), and Pamphagidae (2n male=19,18) are presented.
EN
The genus Isophya represents one of the largest orthopteran genera with about 45 species occurring in Europe. All over its range the genus includes groups of sibling species, the recognition of which is hampered by their morphological similarity. In part, some species were grouped according to their morphology: the Isophya straubei-group, the I. amplipennis-group and the I. major-group. Nevertheless,many species have not been studied using newmethods. This is particularly true for 15 taxa described from the Balkans, more or less resembling I. modesta, known mostly from old, unsatisfactorily informative descriptions. Chromosomal analysis of 25 species/subspecies of the genus Isophya showed the karyotypic evolution among species. The karyotype of 18 species/subspecies was investigated for the first time. The most remarkable changes in the sex chromosomes were the ancient acrocentric X chromosome inversions in 19 species and an X chromosome/autosome mutual tandem translocation, from which the sex determination system neo-XY originated in I. hemiptera. Karyotype differentiation has been less rapid in autosomes than in sex chromosomes, in this case interspecific autosomal differentiation has involved the distribution and quantity of C-heterochromatin as well as the number of NORs. Cytogenetic analysis revealed some changes reflecting the level of genomic organization,andtheresultssuggestsomeassociationofrelatedtaxafrombiogeographicregions.
EN
Seven categories of B chromosomes found in the brachypterus grasshopper Podisma sapporensis from Hokkaido populations differ in structure, size, and C-band content. The interchange between B and one autosome from M3 and sporadically M7 was observed in most of the populations examined. Such an interaction between standard and non-standard chromosomal set provides an insight into the integration of supernumerary chromosome. In addition, C-heterochromatin polymorphism was also identified in male karyotypes in some populations. These facts indicate P. sapporensis is a highly polymorphic species from the cytogenetic point of view.
EN
Karyotypes (chromosome number and shape) of four species of the subtribe Liarina were studied. The chromosome numbers and NF (Fundamental Number) in this group of species range from 2n%=33 (34) to 27(30): Liaromorpha buonluoiensis 2n%=33 (34), Sialaiana transiens 2n%=29 (34), Liara tramlapensis 2n%=29 (32), and Anelytra (Perianelytra) propria 2n%=27 (30). Cyto-taxonomy analysis indicates an intensive karyotype evolution among species belonging to three different groups of the genera. Differences of karyotypes are connected with Robertsonian fusion and tandem fusion in autosomes. Additionally, C-banding distribution and location of the NORs were studied.
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