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Toxicity and stability of silver nanoparticles to the green algaPseudokirchneriella subcapitatain boreal freshwater samples and growth media

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Tuominen M., Schultz E., Sillanpää M.
Nanomaterials and the Environment
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2013
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vol. 1
48-57
EN
The toxicity of silver nanoparticles (AgNPs) to green alga Pseudokirchneriella subcapitata was evaluated in standard nutrient medium (ISO 8692), lake water samples from an oligotrophic and an eutrophic lake, and in lake waters supplemented with the standard nutrient medium. Prior to toxicity testing the agglomeration of polyvinylpyrrolidone (PVP) and starch-coated AgNPs was studied in each test medium. Agglomeration was studied by determining the hydrodynamic diameter (HDD). The HDDs for the PVP- and starch-capped AgNP dispersions in deionized water were 40 and 175 nm respectively, indicating the presence of agglomerates. The HDDs of AgNPs remained stable throughout the exposure time in all media used for the toxicity tests. The algae growth inhibition test was performed as a microplate modification of the ISO method using fluorescence detection. The effect of concentration at a 50% inhibition value for PVPcoated AgNPs in standard medium was 115 ± 3 μg/L, and for starchcoated AgNPs 51 ± 32 μg/L. The eutrophic freshwater conditions suppressed the toxicity of the PVP- coated AgNPs, but not the starchcoated NPs. This finding emphasizes the importance of using different AgNPs and natural waters in assessing the environmental risks of silver nanoparticles.
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Progresses in treatment of collagen and keratin-based materials with silver nanoparticles

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Gaidau C., Petica A., Micutz M., Danciu M., Vladkova T.
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EN
The treatment of chromium tanned sheepskins with poly(hydroxyl urethane) and dispersion of chemically synthesized nanosilver showed the best resistance to fungi and bacterial exposure tests. These collagen-based materials containing different amounts of nanosilver were investigated by Atomic Absorption Spectroscopy, Atomic Force Microscopy and Scanning Electron Microscopy coupled with Energy Dispersive X-Ray Spectroscopy. The influence of sheepskins treated with silver nanoparticles on the wound healing process was assessed and the nanoparticles concentration seems to have a positive effect up to 370 ppm and does not influence the inflammatory process above this concentration.
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