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1

Genomic organization and nucleotide sequence of the repetitive DNA from Vicia sativa L.

100%
Lehmann P., Kozubek E.
Genetica Polonica
|
1993
|
vol. 34
|
issue 3
185-195
EN
The present study refers to the previously reported identification of a family of interspersed repeated sequence in Vicia sativa. It deals with the organisation, and potential of these sequences. A high degree of homology was found between repeats belonging to this family within both Vicia sativa and two other species, which indicates a conservative nature of these repeated sequences. Dot blot hybridization and computer analysis suggest that it is possible to express DNA fragments localized within the repeated sequences of the described family. The family of sequences, the object of these studies, has not been identified in the genome of Vicia sativa so far.
2

Conservation of ferritin amino acid sequences

100%
Smol J.
Biotechnologia
|
1997
|
issue 4
135-139
EN
The present review describes the conservation of ferritin amino acid sequences. We investigated the N-terminal amino acid sequence of lupin ferritin. We found that both subunits had the same N-terminal sequence. Our data confirm that subunits are not programmed by different mRNAs but they are a result of posttranslational modification. The smaller subunit has a lower molecular weight probably because of deletion of some amino acids from the C-terminal end. Then we compared the lupin ferritin sequence with other known ferritin sequences from plants and animals. Sequence data showed that the identity of plant sequences was 60-92,5%. This high sequence similarity was improved by analysis of genomic DNA from maize and soybean. Both DNAs share 72,2% nucleotide sequence identity. An additional plant specific sequence (named the transit sequence) was observed at the N-terminal end. This sequence consists of 50 nucleotides. We found the lupin ferritin did not have that sequence. We suggest that the deletion of the transit sequence in lupin ferritin seems to be the result of isolation and purification.
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