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1

Crystalization of nucleic acids

100%
Adamiak D. A.
Biotechnologia
|
1993
|
issue 4
137-146
EN
Crystalization is the most important and chellenging step in the determination of the structure of biomolecules and macromolecules by diffracton methods.Macromolecules are extremely complex physical-chemical system whose properties vary as a function of many environmental influences such as temperature, pH, ionic strength and solvent composition.Therefore, a number of factors influencing macromolecule crystal growth ought to be considered.Despite of consderable interest in the approaches allowing for continuous monitoring and setting of crystal growth conditions, in practice, numerous trials ought to be performed in order to obtain appropriate crystals characterized by their optimal geometrical parameters,crystallographic symmetry, unit cell and, most importantly,their high resolution.The vapour diffusion and the microdialysis methods are discussed as the most widely used crystallization methods of macromolecules.
2

A comparison of nucleic acid content in Balantidium coli, trophozoites from different isolates

80%
Skotarczak B., Zielinski R.
Folia Biologica
|
1997
|
vol. 45
121-124
EN
Cytophotometric assays were performed on Balantidium coli trophozoites isolated from 30 pigs affected by acute balantidiasis (Group I) and from 30 pigs with symptom-free balantidiasis (Group II). Trophozoites from cultures obtained from Group I and II pig isolates were assayed for comparison. Comparative cytophotometric studies on nucleic acids of B.coli trophozoites isolated from acute and symptomless balantidiasis-affected pigs as well as from in vitro cultured trophozoites showed differences which could have resulted from diferences between populations in the trophozoans under investigation.
3

Effect of tenotomy on extensor digitorum longus muscle in Sprague Dawley rats

80%
Gill N., Shakoori A. R.
Folia Biologica
|
1998
|
vol. 46
109-118
EN
The effect of tenotomy on Extensor Digitorum Longus (EDL) muscle, brought about by removal of anterior tibialis muscle was studied in the rat. Hypertrophy as well as hyperplasia of the muscle fibres were observed following tenotomy of intact muscle. The transplanted muscle regenerated after tenotomy in the form of a flat strap. The weight, length, breadth and total muscle area of intact muscle increased but decreased in transplanted muscle following tenotomy. The number of muscle fibres/microscopic field increased during the entire experiment in intact muscle after tenotomy, while in transplant the number of fibres first decreased and then increased. The diameter of intact and transplanted muscle increased after tenotomy. The number and diameter of nuclei was normalized at the end of experiment in both groups. The DNA and RNA content was increased in intact muscle after tenotomy. In transplant DNA and RNA content first increased but than decreased. The protein content was increased in intact muscle at the end of the experiment but decreased in transplant after tenotomy.
4

Application of hihg pressure in molecular biology and biotechnology

80%
Krzyzaniak A., Jurczaki J., Barciszewski J.
Biotechnologia
|
1995
|
issue 1
148-157
EN
The application of high pressure for biological macromolecules investigations was an object of interest already in the begining of this century.Recently, attention has been paid to this method because of its possible applications in biotechnology.In this paper, we summarized the data existing in the literature on the machanism of high pressure effects on protein and nucleic acids.We also reviewed various practical aplications of high pressure, especially in food industry.
5

Application of high pressure in food technology and medicine

70%
Wandel A., Gensler I., Porowski S., Jurczak J., Barciszewski J.
|
|
issue 1
199-211
EN
For the first time, high pressure was used for milk sterilization at the beginning of this century. However, only recently attention has been paid to this method and its possible applications in food preservation and inactivation of some viruses. In this review, we summarize high pressure effects on biological macromolecules such as proteins, nucleic acids, ribosomes and other cell components. We also show examples of practical application of high pressure in food conservation and medicine.
6

Effectivity of plant genetic transformation systems and proteins-nucleic acids interaction

70%
Ciesielczyk P., Bartkowiak S.
Biotechnologia
|
1995
|
issue 2
52-72
EN
Possible interactions between proteins and nucleic acids which might contribute to general effectivity op plant genetic transformation systems are reviwed. Experimental data concerning direct and A tumefaciens - based plant genetic transformation systems are compared with data obtained from research on nucleocytoplasmic exchange machanisms of biopolmers.In thaht context, mechanisms of hnRNA interactions with hnRNP appear to be of great importance. This type of interactions is determined by conservative nucleic acida bindng domains of many cellular proteins e.g. RGG and RBD.These proteins seem to play an important role in the integration of many other cellular processes.
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