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EN
Sediment samples collected in 1997 and 1998 from the Wislane, Obroncow Poczty Polskiej or Siarkowe (Sulfur), Oliwskie and Paliw Plynnych (Liquid Fuels) quays were sorted into four grain size fractions, i.e., <2.00 mm; 2.00 - 0.063 mm; 0.063 - 0.032 mm; <0.032 mm. It was determined that the sediments in the port area are small-grained sands with a small admixture of silt. The analyzed benthic sediments contained different amounts of organic matter and displayed varying water content. The mean contents of the labile form of lead and its total concentration in the grain size fraction <2.00 mm were 6.4 mg.kg ?1 d.w. and 11.4 mg.kg ?1 d.w., respectively. These values for nickel were 2.9 mg.kg ?1 d.w. for the labile form and 7.1 mg.kg ?1 d.w. for total concentration. The widest range of values was noted in the sediment surface layer (0-20 cm) for total concentration and labile forms. Sediments collected from locations close to the Gulf of Gdansk (e.g., Liquid Fuels Quay) and from areas with low industrial activity (e.g., Sulfur Quay) exhibited Pb and Ni concentrations which were many times lower than those in other areas of the port which were investigated.
EN
Radiochemical procedures are described for the determination of iron 55Fe and nickel 63Ni isotopes in environmental samples. The methods were based on the mineralisation of sediment and biological material, coprecipitation and separation of iron and nickel on anion exchange resin. The separated isotopes were electroplated onto a copper disc and their activi-ties was measured by beta spectrometry using an anti-coincidence GM gas flow counter. The proposed procedures were tested on samples from the Baltic Sea. The concentration of 55Fe in reactor water samples falls within the range from 0.15 to 2185 Bq ?1-1,while in brown algae Fucus vesiculosus it lies between 0.13 and 3.50 Bq ? kg-1 dry wt. The 63Ni is non-uniformly distributed within the Baltic fish. More than 95% of the total 63Ni content in cod and herring is located in fillet with skin and scale.
EN
Experiments were carried out on the effect of nickel as an inorganic compound (NiSO4?7H2O) and organic Ni(II) complexes (i.e. Ni(II)-Glu and Ni(II)-EDTA) in concentrations of 20, 40 and 85 muM dm-3 on meristematic cells of root tips of Brassica oleracea L. cv. S?awa from Enkhouizen. All three tested chemical forms of nickel had a mitodepressive effect and inhibited root elongation. With respect to the degree of root elongation inhibition and mitodepressive effect, the tested forms of nickel can be put in the following order: Ni(II)-Glu NiSO4?7H2O Ni(II)-EDTA. In all three tested forms, nickel caused disturbances in mitotic divisions, resulting in anaphase bridges and binuclear cells, whose nuclei were joined by a bridge of condensed chromatin or separated. Inorganic nickel and Ni(II)-Glu in higher concentrations damaged nuclei (the amount of condensed chromatin increased), nucleoli (their structure became more condensed and vacuolisation was observed), endoplasmic reticulum (fragmentation, swelling of cisternae) and mitochondria (structure condensation).
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