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Cell death and neuronal arborization upon quercetin treatment in rat neurons

100%

Jakubowicz-Gil J., Rzeski W., Zdzisinska B., Dobrowolski P., Gawron A.

Quercetin, one of the major flavonoids, exhibits many beneficial effects on human organism as antihistamine, antioxidant, anti-inflammatory, anticancer and antiviral drug. It is recommended as suplement of healthy diet but still the knowledge of its beneficial effect on normal cells is not satisfactory. We decided to examine the effect of flavonoid on neurons morphology and their susceptibility to cell death. Fractal analysis of rat neurons revealed that 24 hours long incubation with quercetin diminished neuronal arborisation in cortical neurons. Neurons also appeared to be very sensitive to cell death after flavonoid treatment in concentration dependent manner. Over 50% of cells died after incubation with 15 ?g/ml of flavonoid while 1 ?g/ ml of quercetin induced cell death only in 5%. Staining with Hoechst 33342 and propidium ioidide revealed the two types of cell death: apoptosis and necrosis. The number of apoptotic cells was comparable with necrotic ones. These results suggest toxic effect of quercetin on neurons what should be taken into consideration in further studies on using quercetin as therapeutic agent.

Effects of implantation of Alzet 1007D osmotic minipumps upon 2-deoxyglucose uptake in the cerebral cortex of mice

88%

Jablonska B., Goerdalski M., Kublik A., Skangiel-Kramska J., Kossut M.

Acta Neurobiologiae Experimentalis

1993

vol. 53

issue 4

577-580

Phosphatidylserine externalization during bone marrow cell death after treatment of mice with WR-2721 and gamma-rays

75%

Mayur L., Bochenek M., Augustznek A.

Folia Biologica

2002

vol. 50

issue 1-2

95-100

The cell surface exposure of phosphatidylserine (PS) and the plasma membrane impairment were assessed in the bone marrow of adult male Swiss mice exposed to a single 6 Gy dose of 60Co gamma-rays, and treated intraperitoneally with the aminothiol WR-2721 (Amifostine, S-2-/3-aminopropylamino/ethyl phosphorothioic acid), at a dose of 400 mg/kg body weight, 30 min prior to gamma-irradiation. The bone marrow cells were stained with a combination of fluoresceinated annexin V (annexin V - FITC) and propidium iodide (PI) at 3 h, 7 h, and 24 h after treatment of mice with WR-2721 and 60Co gamma-irradiation. The number of early apoptotic cells (annexin V - FITC positive/ PI negative), and late apoptotic and necrotic cells (annexin V - FITC positive/ PI positive), was increased at 3 h after exposure of mice to 60Co gamma-rays and thereafter declined with the frequency of apoptotic and necrotic cells remaining lower in WR-2721 pre-treated mice. Using the annexin V - FITC flow cytometric assay, the radioprotective effect of WR-2721 against induction of apoptosis and necrosis in normal cells of the haematopoietic system was shown.

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2 Acta Neurobiologiae Experimentalis

1 Folia Biologica

1 2008

1 2002

1 1993

1 Augustznek A.

1 Bochenek M.

1 Dobrowolski P.

1 Gawron A.

1 Goerdalski M.

1 Jablonska B.

1 Jakubowicz-Gil J.

1 Kossut M.

1 Kublik A.

1 Mayur L.

1 Rzeski W.

1 Skangiel-Kramska J.

1 Zdzisinska B.

Search results

Cell death and neuronal arborization upon quercetin treatment in rat neurons

Effects of implantation of Alzet 1007D osmotic minipumps upon 2-deoxyglucose uptake in the cerebral cortex of mice

Phosphatidylserine externalization during bone marrow cell death after treatment of mice with WR-2721 and gamma-rays