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DETERMINATION OF THE HYDROLYTIC ACTIVITY OF WHOLE SALIVA USING CHITOSAN ASCORBATE AS A SUBSTRATE

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Kochańska B., Łukaszewska M., Ochocińska J.
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vol. 23
97 - 102
EN
The aim of this work was to evaluate of hydrolytic activity of whole saliva using chitosan ascorbate as a substrate. In this aim, the concentrations of N-acetyl-D-glucosamine were determined in saliva before addition of chitosan ascorbate, directly after addition and during incubation with chitosan ascorbate by 20 hrs. In this in vitro study were used sterile chitosan ascorbate in the form of powder. Chitosan was obtained from krill chitin. The ratio of ascorbic acid to chitosan was 1:1. The unstimulated whole saliva showed the hydrolytic activity in the presence of the chitosan ascorbate as a substrate.
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