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Enantioselective inhibition of immobilized acetylcholinesterase in biosensor determination of pesticides

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Kaniewska M., Jońca J., Połeć I., Sikora T., Marty J.-L., Trojanowicz M.
Open Chemistry
|
2012
|
vol. 10
|
issue 6
1760-1765
EN
Chiral effects for the inhibition of acetylcholinesterase by organophosphorus pesticides were investigated for insecticide malathion and malaoxon, which is a metabolic product of malathion in living organisms. Studies were carried out using a bienzymatic biosensor with immobilized acetylcholinesterase, choline oxidase, and with Prussian Blue used as a mediator. In both cases the R enantiomers accelerate acetylocholinesterase inhibition. The chiral effect in inhibition was much more pronounced in fast flow measurements than in batch measurements. [...]
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