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Cytotoxic Activity of Paris quadrifolia Extract and Isolated Saponin Fractions Against Human Tumor Cell Lines

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Stefanowicz-Hajduk J., Kawiak A., Gajdus J., Ochocka J., Paszkiewicz M., Stepnowski P., Łojkowska E.

Acta Biologica Cracoviensia s. Botanica

2011

vol. 53

issue 2

Cross-resistance to five glucocorticoids in childhood acute lymphoblastic and non-lymphoblastic leukemia samples tested by the MTT assay: Preliminary report.

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Styczyński J., Wysocki M., Dębski R., Balwierz W., Rokicka-Milewska R., Matysiak M., Balcerska A., Kowalczyk J., Wachowiak J., Sońta-Jakimczyk D., Chybicka A.

2002

vol. 49

issue 1

93-98

In vitro antileukemic activity of five glucocorticoids and their cross-resistance pattern in childhood acute lymphoblastic and non-lymphoblastic leukemia were determined by means of the MTT assay in 25 leukemia cell samples of childhood acute leukemias. The equivalent antileukemic concentrations of the drugs tested were: 34 μM hydrocortisone (HC), 8 μM prednisolone (PRE), 1.5 μM methylprednisolone (MPR), 0.44 μM dexamethasone (DX) and 0.22 μM betamethasone (BET). In comparison with initial ALL cell samples, the relapsed ALL group was more resistant to PRE (38-fold, p = 0.044), DX (> 34-fold, p = 0.04), MPR (38-fold), BET (45-fold) and HC (33-fold). The AML cell samples were even more resistant to: PRE (>85-fold, p=0.001), DX (> 34-fold, p = 0.004), MPR (> 69-fold, p = 0.036), BET (> 69-fold, p = 0.038) and HC (54-fold, p = 0.059) when compared with ALL on initial diagnosis. A significant cross-resistance among all the glucocorticoids used was found. Only in some individual cases the cross-resistance was less pronounced.

Epicatechin-copper 9II) complexes: Damage of small intestinal epithelium

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Stavrescu R., Kimura T., Hayakawa F., Ando T.

Four epicatechins [(−)-epicatechin (EC), (−)-epicatechin gallate (ECg), (−)-epigallocatechin (EGC), (−)-epigallocatechin gallate (EGCg)] and their corresponding copper complexes were compared with regard to their effect on the viability of Caco-2 colon cancer cells in vitro, measured by 3-(4,5-dimethylthyazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay. The viability of Caco-2 cells exposed to EC (1 mM), ECg (1 mM) or EGC (1mM) respectively, for 30 min, was comparable to that of the saline control group, while EGCg (1 mM) apparently enhanced cellular activity. in contrast, the cells treated with epicatechin-copper complexes were killed. Bivalent copper 91 mM), in similar conditions, did not affect the cells. No cell leakage or other histological differences were observed, implying a rapid cell death. The suggested mechanism of killing is by OH radical attack, produced in the presence of epicatechin-copper complexes, but not in the presence of either of the epicatechins or copper alone. The reaction sites are discussed.

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1 Acta Biochimica Polonica

1 Acta Biologica Cracoviensia s. Botanica

1 Open Chemistry

1 2011

1 2003

1 2002

1 Ando T.

1 Balcerska A.

1 Balwierz W.

1 Chybicka A.

1 Dębski R.

1 Gajdus J.

1 Hayakawa F.

1 Kawiak A.

1 Kimura T.

1 Kowalczyk J.

1 Matysiak M.

1 Ochocka J.

1 Paszkiewicz M.

1 Rokicka-Milewska R.

1 Sońta-Jakimczyk D.

1 Stavrescu R.

1 Stefanowicz-Hajduk J.

1 Stepnowski P.

1 Styczyński J.

1 Wachowiak J.

1 Wysocki M.

1 Łojkowska E.

Search results

Cytotoxic Activity of Paris quadrifolia Extract and Isolated Saponin Fractions Against Human Tumor Cell Lines

Cross-resistance to five glucocorticoids in childhood acute lymphoblastic and non-lymphoblastic leukemia samples tested by the MTT assay: Preliminary report.

Epicatechin-copper 9II) complexes: Damage of small intestinal epithelium