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Patterned growth and differentiation of human cord blood- derived neural stem cells on bio-functionalized surfaces

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Buzanska L., Ruiz A., Zychowicz M., Rauscher H., Ceriotti L., Rossi F., Colpo P., Domanska-Janik K., Coecke S.

Acta Neurobiologiae Experimentalis

2009

vol. 69

issue 1

24-36

Bio-functionalized surfaces were prepared to study the adherence and differentiation capacity of neural stem cells derived from human umbilical cord blood (HUCB-NSC). Cell growth platforms containing arranged arrays of adhesive molecules were created by microcontact printing on a biologically inert surface. Biomolecules used to prepare microarray platforms included the extracellular matrix protein fibronectin and the polyaminoacid poly-L-lysine. HUCB-NSC plated on microplatforms at various serum conditions showed serum and molecule type dependent capacity for adhesion and differentiation. Poly-L-lysine allowed the maintenance of stem-like non differentiated cells attached to the surface, whereas fibronectin promoted spreading and neural commitment. Serum deprivation did not influence the attachment of HUCB-NSC to fibronectin, but significantly enhanced the attachment to poly-L-lysine and promoted dBcAMP induced neuronal differentiation. A bio-pattern of squares with interconnecting lines was used to guide neuronal differentiation by directing cell protrusion outgrowth. Tailoring the geometry of the bio-pattern enabled directing and monitoring of the neural stem cells. development in the large scale multiparameter biotests.

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1 Acta Neurobiologiae Experimentalis

1 2009

1 Buzanska L.

1 Ceriotti L.

1 Coecke S.

1 Colpo P.

1 Domanska-Janik K.

1 Rauscher H.

1 Rossi F.

1 Ruiz A.

1 Zychowicz M.

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Patterned growth and differentiation of human cord blood- derived neural stem cells on bio-functionalized surfaces