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The modulatory effect of aminothiols on the clastogenic activity of X-rays assayed by the in vivo mouse micronucleus test

100%

Mazur L.

Folia Biologica

1996

vol. 44

55-59

The modulatory effect of AET (S-2-aminoethylisothio-uronium bromide hydrobromide) and WR-2721 (S-2-/3-ami- nopropylamino/ethylphosphorothioic acid) on the clastogenic activity of X-rays was assessed by the in vivo mouse micronucleus test. The frequency of micronucleated polychromatic erythrocytes (MNPCEs) in the peripheral blood of adult male Swiss mice exposed to 5 Gy X-rays alone, or treated with AET or WR-2721, at a dose of 200 mg/kg body weight, 15 or 30 minutes prior to X-irradiation, respectively, was determined during a fifteen-day period. The number of micronuclei increased on day 1 post-irradiation in X-irradiated mice and declined thereafter with the frequency of MNPCEs remaining lower in the thiol pre-treated mice. A more effective protection against the clastogenic activity of X-rays in the erythropoietic system was observed after WR-2721 administration than AET application.

DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test

75%

Brozovic G., Orsolic N., Rozgaj R., Kasuba V., Knezevic F., Knezevic A. H., Benkovic V., Lisicic D., Borojevic N., Dikic D.

vol. 51

issue 1

79-86

The relationship between DNA damage and repair of peripheral blood leukocytes, liver, kidney and brain cells was investigated in Swiss albino mice (Mus musculus L.) after exposure to sevoflurane (2.4 vol% for 2 h daily, for 3 days). Genetic damage of mouse cells was investigated by the comet assay and micronucleus test. To perform the comet assay, mice were divided into a control group and 4 groups of exposed mice sacrificed on day 3 of the experiment, at 0, 2, 6 or 24 h after the last exposure to sevoflurane. Mean tail length (TL), tail moment (TM), and tail intensity (TI) values were significantly higher in exposed mice (all examined organs) than in the control group. Significant DNA damage immediately after exposure to sevoflurane was observed in leukocytes. Damage induction in the liver, kidney, and brain occurred 6 h later than in leukocytes, as expected according to the toxicokinetics of the drug, where blood is the first compartment to absorb sevoflurane. However, none of the tested tissues revealed signs of repair until 24 h after the exposure. To distinguish the unrepaired genome damage in vivo, the micronucleus test was applied. Number of micronuclei in reticulocytes showed a statistically significant increase, as compared with the control group at all observed times after the treatment.

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1 Folia Biologica

1 Journal of Applied Genetics

1 2010

1 1996

1 Benkovic V.

1 Borojevic N.

1 Brozovic G.

1 Dikic D.

1 Kasuba V.

1 Knezevic A. H.

1 Knezevic F.

1 Lisicic D.

1 Mazur L.

1 Orsolic N.

1 Rozgaj R.

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The modulatory effect of aminothiols on the clastogenic activity of X-rays assayed by the in vivo mouse micronucleus test

DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test