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The evolution of biosensor research corresponds with the increasing need for fast, sensitive and selective measuring device.This paper will focus on recent developments that have been advanced in our Institute. (I) As an electrochemical device a highly sensitive multi-enzyme sensor for the determination of inorgatic phosphate was developed using 4 enzymes for signal amplification. (II) Immunosensing of low molecular mass analytes is demonstrated by a flow injection system for the determination of herbicides in drinking water, such as 2,4-D and for the determination of polycyclic aromatic hydrocarbons in soil. (III) Microbial sensors for the determination of polycyclic aromatic hydrocarbons were developed as well as new approaches to the determination of the biochemical oxygen demand.(BOD). (IV) The "double matrix membrane" technology is an innovative possibility for the production of cheap disposable electrodes for sensors, for example for the detection of urea.(V) Based on surface plasmon resonance spectroscopy (SPRS) a miniaturized fibre optical sensor was developed for sensing environmental hazards in liquids as well as in air.
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