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Biotechnologia
|
2009
|
issue 1
119-131
EN
Over the past few years, Medicago truncatula has emerged as a model legume for the study of plant functional genomics that incorporates profiling of gene expression (transcriptome), protein expression (proteome), and metabolite expression (metabolome) to better understand the biological processes with legumes and their interaction with the environment. It is an excellent species for fundamental studies on the unique secondary metabolism of legumes. Two classes of secondary metabolites are of particular interest: phenylopropanoids, which have been attributed with health-promoting properties, And another important class of secondary metabolites from M. truncatula are the triterpene saponins, which possess allelopathic, anti-microbial, anti-insect activity, as well as anti-nutritional effects. Profiling and identification of a large variety of natural products from M. truncatula are crucial to metabolomics and functional genomics.
EN
The concept of metabolomic studies has been developed during the last decade. Comprehensive analysis of primary and secondary metabolites in living organisms grown under genetic and environmental stresses may deliver interesting information about the status of the studied microorganisms, plants or animals. The metabolome analysis may be performed using different methodical approaches and various physicochemical methods may be applied for identification of low molecular compounds. Due to huge sets of data collected during the metabolomic studies, different statistical calculations and bioinformatic technologies are used for the presentation and interpretation of the results.
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