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Comparison of micropropagation methods in Polish cultivars of barley and oat

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Kruczkowska H., Pawlowska H., Skucinska B.
Biotechnologia
|
2010
|
issue 2
83-88
EN
Obtaining transgenic plants in cereal cultivars of agronomic value requires an efficient method of micropropagation which does not cause additional variation. Recently suggested protocols consist in inducing regeneration of multiple shoots from apical meristems of seedlings derived in vitro from mature dry seeds. We have compared the efficiency of three protocols: after Zhang et al. (4), Sharma et al. (8) and Ganeshan et al. (10) in several Polish cultivars of barley and oat. I In the examined cultivars, the protocol after Ganeshan et al. (10) proved to be more efficient, less laborious and faster.
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