Intergeneric hybridization was carried out between various accessions of Hordeum jubatum (4x) and cultivars of Triticum aestivum (6x) and Triticale (6x) as well as T.monococcum (2x) and between cultivars of Triticum aestivum (6x) and the hybrid (Horodeum jubatum 4x X Secale cereale 4x) in both directions.The hybrid progeny was obtained via embryo culture from crosses of H.jubatum x T.aestivum and T.aestivum x(H.jubtum 4x X S.cereale 4x).The hybrid of H.jubatum x T.aestivum was produced at the frequency of 1.7% in relation to pollinated florets and five hybrids of T.aestivum x (H.jubatum 4x x S.cereale 4x) from successful combinations were produced at the frequency of 2.00-4.88%.The hybrids exibited varitionin somatic chromosome number.In meiocytes of two T.aestivum x (H.jubatum 4x X S.cereale 4x) plants a high chromosome instability was also found.The chromosomes were associated mostly as univalents, but some pairings (0.03-3.50 per cell) mainly as rod bivalents were observed.
In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations.Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation.The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants).Plants obtained vie embryo callus culture showed good vegetative vigour and well-developed root system.Spike morphology of all plants resambled that of rye.Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The hetromorphic bivalent-like configurations occured in five plants in 0.01-0.02 per cell.The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with the chiasm frequency of 9.36-17.75 per cell.All plants, with 14 and 28 chromosomes, were completly sterile both in backcrosses and when selfed.
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