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1

Plant growth regulators and somatic embryogenesis in Medicago sativa L.

100%
Kepczynski J., Kepczynska E.
Biotechnologia
|
2001
|
issue 2
16-25
EN
Somatic embryogenesis, resembling zygotic embryogenesis in vivo, is considered to be an efficient method of in vitro propagation of a number of agronomically important plant species including Medicago sativa L. and it offers an in vitro experimental system for studying the embryo development. Artificial seed technology is one of the important applications of the process. Induction of embryogenesis, embryo development and induction desiccation tolerance are affected by plant growth regulators. The review will focus on the effect of plant growth regulators: auxins, cytokinins, gibberellins, abscisic acid, jasmonates, ethylene and inhibitors on the synthesis and action of different phases of somatic embryogenesis in Medicago sativa L.
2

The effect of salicylic acid, jasmonates biosynthesis inhibitor, on cell suspension growth, development and regeneration of somatic embryos in Medicago sativa L. tissue cultures

100%
Kepczynska E., Zielinska S.
Biotechnologia
|
2004
|
issue 2
114-121
EN
The effect of salicylic acid (SA) applied during the proliferation phase of cell suspension in vitro regeneration system of Medicago sativa L. on cell suspension growth, production of somatic embryos and their following development, germination and conversion were examined. SA is a potent inhibitor of the above processes. It is suggested that endogenous jasmonates are of some importance in regulation of somatic embryogenesis.
3

Alfalfa (Medicago sativa) as an object for transformation by Agrobacterium tumefaciens and in vitro regeneration

86%
Lisova O., Legocki A. B., Legocki A. B.
Biotechnologia
|
2002
|
issue 3
74-90
EN
From the economical point of view alfalfa is a very valuable plant. It has very high yield potential, it is rich in protein, vitamins and minerals, so it is prized as very important feed for horses, beef cattle, sheep, goats and other domestic animals. That is why the improving of alfalfa quality by the methods of molecular biotechnology is a very interesting issue. In this review information about the crucial elements of alfalfa transformation and regeneration are gathered together. Among other following elements, genetic background of plant, A. tumefaciens strains, explant types, in vitro culture medias, plant development stages are discussed. It seems that optimalisation of the transformation and regeneration procedure are individual for every plant genotype and A. tumefaciens strain.
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