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1

The use of cell lines and the methods of cell cycle synchronization in mammalian cloning

100%

Modlinski J., Karasiewicz J.

Biotechnologia

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1998

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issue 2

86-100

EN

Studies aimed at improving the effectiveness of cloning by nuclear transfer have shown that proper development of the majority of reconstituted embryos is secured by G1 phase of the donor nucleus or by using 'universal recipients' i.e. enucleated pre-activated oocytes. Donor nuclei for cloning may by derived from cell lines of embryonic stem cells (mouse), embryonic cells after short in vitro culture (sheep, pig, cattle) or even from fetal cells or adult cells after in vitro culture and inducing quiescence in their nuclei (sheep). The use of fetal cells for transgenesis in vitro and the production of transgenic sheep after nuclear transfer from these cells opens the way to profitable technology of cloning transgenic farm animals.

2

Activation of Salmonella-specific immune responses in the intestinal mucosa

80%

Srinivasan A., McSorley S. J.

Archivum Immunologiae et Therapiae Experimentalis

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2006

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vol. 54

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issue 1

25-31

EN

The mammalian immune response to Salmonella has long been a subject of scientific study. Indeed, many of the general aspects of bacterial pathogenesis and host immune defense have been well described. However, a lack of clarity remains concerning important aspects of the host immune response to Salmonella, particularly with regard to the induction of an immune response in the intestinal mucosa. A major limitation has been the general lack of knowledge about specific antigenic targets that are recognized by both the innate and adaptive immune response in the intestine. Progress towards the identification of these targets is critical for the development of a detailed model of immunity to Salmonella and will lead to a better understanding of mucosal immune responses to other intracellular pathogens.

3

Cryoconservation of mammalian embryos and oocytes

80%

Gajda B., Smorag Z.

Biotechnologia

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1998

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issue 2

10-33

EN

This paper presents current methods of embryo and oocyte cryoconservation in the following species: mice, rabbits, sheep and goats, pigs, horses and cows. Both the freezing and vitrification methods are discussed with special emphasis on the major factors affecting the efficiency of these two methods.

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2 Biotechnologia

1 Archivum Immunologiae et Therapiae Experimentalis

1 2006

2 1998

1 Gajda B.

1 Karasiewicz J.

1 McSorley S. J.

1 Modlinski J.

1 Smorag Z.

1 Srinivasan A.

The use of cell lines and the methods of cell cycle synchronization in mammalian cloning

Activation of Salmonella-specific immune responses in the intestinal mucosa

Cryoconservation of mammalian embryos and oocytes