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  1. 21 Archivum Immunologiae et Therapiae Experimentalis

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  1. 3 Zak-Nejmark T.

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1

Analysis of chromosome aberrations, sister chromatid exchanges (SCE) and cell division kinetics in human lymphocytes exposed in vitro to new monophosphates of pyrimidine acyclonucleosides

100%
Ferenc T., Rutkowski M., Bratkowska W., Hubner H., Draminski M.
Journal of Applied Genetics
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1998
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vol. 39
|
issue 1
113-127
EN
Five newly synthesised monophosphates of two pyrimidine acyclonucleoside series, namely 1-N-[(2'-hydroxy)ethoxymethyl] and 1-N-[(1',3'-dihydroxy)-2'-propoxymethyl] derivatives of 5- and 5,6-alkylated uracils were tested in vitro for chromosome aberrations and sister chromatid exchanges (SCE). Metaphase plates were obtained via microculture of human lymphocytes from heparinized peripheral blood. The compounds were tested in doses: 10, 20, 40, 80 and 150 ?g per mL of culture. The tested compounds induced mainly chromatid gaps, less frequently chromosome gaps. A low number of mitoses with chromatid and chromosome breaks, acentric fragments, dicentric chromosomes and exchange figures were also observed. The tested compounds in doses: 40, 80 and 150 ?g per mL, doubled or tripled the percentage of cells with chromatid gaps and chromosome gaps as compared to the control. The percentage of cells with aberrations (excluding gaps) induced by the tested compounds in all doses did not exceed 2%. The tested compounds induced a higher number of SCE per cell but less than double frequency as compared to the control. SCE frequencies and replication index (RI) values varied depending on the examined compounds. For the highest dose of the tested compounds (150 ? per mL) a significant decrease in RI values was observed for 1-N-[(2'-hydroxy)ethoxymethyl]-5,6-tetramethyleneuracil monophosphate and for 1-N-[(2'-hydroxy)ethoxymethyl] -5,6-dimethyluracil monophosphate. So far, the results have indicated potential clastogenicity of all the tested compounds except acycloguanosine monophosphate.
2

Lymphocytes distribution and intrahepatic compartmentalization during HCV infection: a main role of MHC-unrestricted T cells

100%
Agrati C., Nisii C., Oliva A., D?Offizi G., Montesano C., Pucillo L. P., Poccia F.
Archivum Immunologiae et Therapiae Experimentalis
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2002
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vol. 50
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issue 5
307-316
EN
Hepatitis-C virus (HCV) infection induces an acute and chronic liver inflammation through an immune mediated pathway that may lead to cirrhosis and liver failure. Indeed, HCV-related hepatitis is characterized by a dramatic lymphocyte infiltrate in the liver which is mainly composed by HCV non-specific cells. Several data indicated that IFN-gamma secretion by intrahepatic lymphocytes (IHL) may drive non specific cell homing to the liver inducing IP-10 production. An interesting hallmark of these IHL is the recruitment of lymphocytes associated with mechanism of innate immunity such as NK, NKT and gamma delta T lymphocytes. CD81 triggering on NK cell surface by the HCV envelope glycoprotein E2 was recently shown to inhibit NK cell function in the liver of HCV-infected persons resulting in a possible mechanism contributing to the lack of virus clearance and to the establishment of chronic infection. In contrast, intrahepatic NKT cells restricted to Cd1d molecules expressed on the hepatocyte surface may contribute to a large extent to the liver damage. Finally, an increased frequency of T cell expressing the gamma delta TCR was observed in HCV-infected liver and recent observations indicate that intrahepatic gamma delta T cell activation could be directly induced by the HCV/E2 particle through CD81 triggering. These cells are not HCV specific, are able to kill target cells including primary hepatocytes and their ability to produce Th1 cytokines is associated with an higher degree of liver disease. Altogether, CD1d/NKT and/or E2/CD81 interactions may play a major role in the establishment of HCV immunopathogenesis. In absence of virus clearance, the chemokine-driven recruitment of lymphocytes with an innate cytotoxic behavior in the liver of HCV infected patients may boost itself leading to the necroinflammatory and fibrotic liver disease.
3

Abnormal distribution of gamma delta T lymphocytes in Graves' disease and insulin-dependent diabetes type

100%
Kretowski A., Mysliwiec J., Kinalska I.
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vol. 48
|
issue 1
39-42
EN
There is an increasing evidence that CD3+ cells, bearing gamma delta T cell receptors representing a minor subpopulation of T cells in the peripheral blood of humans are involved in the development of autoimmunity. The aim of the present study was determination of the gamma delta T cell subpopulation levels in the peripheral blood of subjects with Graves' disease and newly diagnosed type 1 diabetes in comparison to age-matched healthy controls. The percentages of CD3+, CD8+, gamma delta TCR+CD8+, gamma delta TCR+CD8? lymphocyte subsets were measured by flow cytometry. In the peripheral blood of newly diagnosed Graves' disease patients we showed a significant decrease of gamma delta TCR+ cells and gamma delta TCR+CD8? subset content in comparison to the percentages observed in subjects after methimazole treatment and in healthy controls. We also found a significant increase of TCR+CD8+ cells in the peripheral blood of subjects with insulin-dependent diabetes, treated with insulin for 3?6 months. The present findings confirm our previous hypothesis that gamma deltaTCR+CD8+ lymphocyte subset could play a role in the pathogenesis of diabetes type 1, probably as regulatory T cells and could be induced by delivery of exogenous insulin. Our results suggest that gamma deltaT cells (gamma delta TCR+CD8? subset) could also play an important role in the development of Graves' disease and that their levels are modulated by thyreostatic treatment.
4

The influence of calyculin A on lymphocytes in vitro

100%
Kowalska A., Srebniak M., Wawrzkiewicz A., Kaminski K.
Journal of Applied Genetics
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2003
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vol. 44
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issue 3
413-418
EN
Human lymphocytes were cultured in vitro and treated with calyculin A. The aim of this work was to estimate the influence of calyculin A on chromosome morphology and banding patterns. It was also interesting whether calyculin A treatment is useful in cytogenetic analysis of human karyotype. We proved that calyculin A induces chromosome condensation in lymphocytes and raises the mitotic index significantly. Moreover, calyculin A does not influence the banding patterns. Therefore it is concluded that calyculin A can be clinically useful for human karyotyping.
5

TCR alpha beta+ CD8+ and TCR gamma delta lymphocytes inhibit the capability of peritoneal macrophages to induce contact hypersensitivity

100%
Majewska M., Zajac K., Zemelka M., Sura P., Gryglewski A., Szczepanik M.
Folia Biologica
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2009
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vol. 57
|
issue 1-2
23-27
EN
Macrophages (Mf) play an important role in induction and regulation of the immune response. It was shown previously that subcutaneous injection of hapten conjugated macrophages (TNP-Mf) induces the contact hypersensitivity (CHS) response, whereas intravenous (i.v.) or intraperitioneal administration of TNP-Mf results in unresponsiveness as a result of induced T suppressor (Ts) cells. The aim of this study was to determine if different T cell populations influence macrophages to become inducers of immunological suppression. Our findings show that indeed i.v. injection of TNP labeled macrophages isolated from control mice into syngenic recipients induces unresponsiveness. However, i.v. administration of TNP substituted macrophages isolated from TCR'-/-, TCR*-/- and $2m-/- mice induces strong CHS similar to that observed after skin painting with TNP-Cl.Moreover, it was shown that TNP conjugated macrophages isolated from CD1d-/- mice were still able to promote immunosuppression when injected intravenously. This suggests that TCR'$+ CD8+ and TCR(*+ lymphocytes stimulate macrophages to induce immunosuppression instead of a strong CHS reaction, whereas CD1d dependent NKT cells are not involved in negative regulation of macrophage function.
6

A study on influence of different signs of air-ions on sister-chromatid exchange frequency and chromosome aberrations in human peripheral blood lymphocytes

100%
Wiszniewski A., Kretowicz T.
Journal of Applied Genetics
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1999
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vol. 40
|
issue 2
129-134
EN
A cytogenetic analysis of peripheral blood lymphocytes of six persons (seven in analysis of chromosome aberrations (CA)) was carried out. The cell cultures were exposed to a constant concentration of positive or negative ions. The exposure time was established as 24 and 72 hours for ions of both signs. In the cultures exposed to positive ions and used for sister-chromatid exchange (SCE) analysis no metaphases were detected. An analysis of cultures after 72 hours of exposition to negative ions revealed an increase in the number of SCE and inhibition of cellular divisions as compared to the control. In CA analysis no differences in the number of aberrations were observed but in cultures exposed to positive ions dicentric chromosomes were detected. Therefore we conclude that the different signs of air-ions had not genotoxic effect.
7

Tracking lymphocytes in vivo

100%
Adams C. L., Kobets N., Meiklejohn G. R., Millington O. R., Morton A. M. Y., Rush C. M., Smith K. M., Garside P.
Archivum Immunologiae et Therapiae Experimentalis
|
2004
|
vol. 52
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issue 3
173-187
EN
The ability to track antigen (Ag)-specific lymphocyte populations in vivo has greatly increased our understanding of the location and functional status of these cells throughout the course of an immune response. Recent technical advances have enhanced researchers' capability to follow migration, activation and cellular interactions of Ag-specific lymphocytes in situ. It is now possible to monitor changes in T cell subsets, co-stimulatory molecules, and chemokine expression within the physiological context of secondary lymphoid organs. Furthermore, the Ag-presenting cell-T cell interaction can be studied, thus dissecting the role and timing of Ag presentation of particular dendritic cell subsets in the initiation of the immune response. The capacity to adoptively transfer small populations of Ag-specific T lymphocytes has also increased our knowledge of the physiologically important role of regulatory T cells in autoimmunity and immunosuppression. New fluorescence imaging techniques such as multicolor video microscopy, laser scanning cytometry, and multiphoton tissue imaging have provided new ways in which researchers can track cellular changes within Ag-specific lymphocytes in vivo. This review summarizes some of the ways in which these techniques have led to discoveries in the role of signaling cascades, cell cycle progression, and apoptosis in maintaining an Ag-specific immune response.
8

Specific immunotherapy stimulates the binding of histamine H2 receptor antagonist by lymphocytes

100%
Zak-Nejmark T., Malolepszy J., Nadobna G., Kuczynska-Sekieta K., Marczynska-Sarul E., Jutel M.
|
|
vol. 40
|
issue 2
113-116
EN
The binding of antagonists of histamine receptors H1 (ranitidine) by perpheral blood lymphocytes from pollinotics was determined before and after the course of immunotherapy. We found that lymphocytes from atopic subjects showed significant decrease in the binding of H2 receptor antagonist as compared to control subjects. specific immunotherapy induced statistically significant increase in H2 receptor antagonist binding, which correlated with the improvement of clinical symptoms.
9

V gene replacement in T and B lymphocytes: illicit or regimented rearrangement?

100%
Golub R.
|
|
issue 4
255-262
EN
Lymphocyte can undergo novel types of secondary genetic rearrangements that alter the specificity of a pre-existing B cell receptor (BCR) or T cell receptor (TCR). V gene replacement is one of them and it replaces an already rearranged V gene segment with an upstream germline V gene segment. This review focuses on the molecular aspect of rearrangement. The role of this mechanism in the immune system is debated.
10

Relationship between impaired apoptosis of lymphocytes and distribution of dendritic cells in peripheral blood and synovial fluid of children with juvenile idiopathic arthritis

100%
Smolewska E., Cebula B., Brozik H., Stanczyk J.
Archivum Immunologiae et Therapiae Experimentalis
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2008
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vol. 56
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issue 4
283-289
EN
Introduction: The pathogenesis of juvenile idiopathic arthritis (JIA) is not fully understood. Recently the present authors described disturbed apoptosis of JIA lymphocytes in both peripheral blood (PB) and synovial fluid (SF) as well as an abnormal distribution of blood dendritic cells (BDCs) between the PB and SF in this disease. Possible relationships between these events during the development of JIA process are assessed here. Materials and Methods: Lymphocyte apoptosis and BDC counts were assessed in the PB and SF of untreated JIA children. Lymphocyte apoptosis was analyzed by the Annexin-V/propydium iodide assay. Total DC (TDC) number was based on the sum of three BDC subpopulations determined using a panel of monoclonal antibodies against BDC antigens (BDCA): myeloid type 1 (mDC1, BDCA-1+/HLA-DR+/CD19?), myeloid type 2 (mDC2, BDCA-3+/HLA-DR+/CD14?), and plasmacytoid (pDC, BDCA-2+/HLA-DR+/CD123+). Cells were enumerated by the flow cytometric ?single-platform' method. The concentration of tumor necrosis factor (TNF)- alpha and the distribution of particular lymphocyte subtypes in both PB and SF were also investigated. Results: There was significant positive correlation between apoptosis of PB lymphocytes and SF TDC count (p=0.002) as well as SF TNF- alpha concentration (p=0.007). SF TNF-alpha levels also correlated with SF TDC count (p=0.003). Moreover, JIA SF was distinctly enriched with CD4+ and CD8+ T lymphocytes and included CD4+/CD25high cells as well. There was significant positive correlation between the number of CD4+/CD25high cells and SF JIA BDC count (p=0.015). Conclusions: These data suggest a possible link between impaired apoptosis of PB/SF lymphocytes and increased recruitment of PB BDCs to SF and other elements of the immune system in JIA, including regulatory CD4+/CD25high cells.
11

Circadian variations of histamine binding to lymphocytes and neutrophils and skin reactivity to histamine in atopic and healthy subjects

100%
Zak-Nejmark T., Nowak I. A., Kraus-Filarska M.
Archivum Immunologiae et Therapiae Experimentalis
|
2006
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vol. 54
|
issue 4
283-287
EN
Intruction: Numerous pathophysiological conditions change during 24-hour periods. Histamine, the main mediator in allergic reactions, exerts a multiplicity of pathophysiological actions through binding to specific receptors on effector cells. Nocturnal exacerbation of symptoms occurs in many atopic diseases in which histamine is an important mediator. Nocturnal wheezing is a very common symptom of asthma. The aim of this study was to determine whether the binding of (fluorescein-labeled) histamine to cells participating in allergic-inflammatory processes (lymphocytes, neutrophils) and skin reactivity to histamine undergo circadian changes and to compare these phenomena in atopic asthmatic and healthy subjects. Materials and Methods: Blood samples were collected at 8 am, 2 pm, 8 pm, 2 am, and 8 am the next day. Histamine skin-prick tests were performed at the same times. Results: It was found that skin reactivity to histamine (wheal, erythema) in healthy subjects underwent significant circadian changes with acrophase at 8 am (wheal) or 8 pm (erythema), the lowest values being at night (2 am, p=0.017), in contrast to atopics, in whom the highest reactivity was found at night (2 am, p=0.002). Significant differences in the binding of fluorescein-labeled histamine between day (8 am?2 pm) and night (2 am) were observed for lymphocytes (p=0.006) and neutrophils (p=0.018). Conclusions: In the asthmatic group these changes were not significant. Circadian changes in both the binding of histamine by effector cells and skin reactivity to histamine were different in healthy and asthmatic subjects, and this may play a role in the pathomechanism, course, and chronopharmacotherapy of atopic diseases.
12

Glucose transport in human peripheral blood lymphocytes influenced by type 2 diabetes mellitus

100%
Piatkiewicz P., Czech A., Taton J.
|
EN
Introduction: The objective of this study was to evaluate glucose transport into lymphocytes in healthy subjects and patients with type 2 diabetes mellitus (DM) treated either with diet only or with insulin and to propose peripheral blood lymphocytes as a convenient model for cellular glucose transport studies. Materials and Methods: Sixty subjects with type 2 DM, 30 treated with diet only and 30 with insulin, were investigated. Thirty healthy subjects matched for age, weight, and sex served as a control group. Deoxy-D-glucose, 2-[3H(G)] transport was studied in isolated peripheral blood lymphocytes. Expression of glucose transporters was ascertained by immunocytochemical identification and by Western blotting. Results: In lymphocytes from the control group, deoxy-D-glucose uptake increased gradually with the duration of the experiment. In diabetics treated with insulin, the maximal increase in deoxy-D-glucose uptake was observed after 30 min of the investigation, followed by a plateau phase. In diabetics treated with diet, deoxy-D-glucose uptake increased slowly during the first 30 min. The presence of GLUT1 and GLUT3 in lymphocytes was confirmed in this study. Conclusions: Glucose transport into lymphocytes is altered in type 2 DM. In lymphocytes from diabetics, the dynamics of deoxy-D-glucose uptake significantly differed from that in healthy subjects. There was also a significant difference between the diabetic groups, representing different modes of therapy and stages of the disease. Glucose transport into lymphocytes is apparently influenced by DM as well as by the mode of therapy. We suggest that peripheral blood lymphocytes may become a promising model for studies on glucose transport in diabetes.
13

Elevated TGF-beta1 concentration in bronchoalveolar lavage fluid from patients with primary lung cancer

100%
Domag?a-Kulawik J., Hoser G., Safianowska A., Grubek- J. H., Chazan R.
Archivum Immunologiae et Therapiae Experimentalis
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2006
|
vol. 54
|
issue 2
143-147
EN
Introduction: Transforming growth factor (TGF)-beta is one of numerous inhibitory factors produced by cancer cells that regulate antitumor immunity. The aim of this study was to evaluate TGF-beta1 levels and lymphocyte subsets in the broncholaveolar lavage fluid (BALF) of patients with primary lung cancer and to analyze the interdependence of these parameters. Materials and Methods: BALF samples were collected from 38 patients with primary lung cancer prior to treatment and from 23 healthy volunteers. Concentrations of TGF-beta1 were measured in two independent lots of samples using a commercially available sandwich ELISA kit after concentration of the supernatants. Differential cell counts in the BALF were performed on slides stained with the May Grnwald Giemsa method. Flow cytometry with monoclonal antibodies was applied for lymphocyte phenotyping. Results: A higher level of TGF-beta1 in the BALF of patients compared with the healthy subjects was observed in both lots of samples (3.232.96 pg/ml vs. 1.050.95 pg/ml, p<0.05, and 16.119.3 pg/ml vs. 10.111.1 pg/m,, respectively, difference not significant). There was significant positive correlation of the TGF-beta1 level with the proportion of lymphocytes and negative correlation with both the proportion of macrophages and the percentage of cytotoxic and activated T lymphocytes. Conclusions: Our findings confirmed that TGF-beta takes part in the local response in the course of primary lung cancer.
14

Histamine receptor expression on peripheral blood lymphocytes is influenced by specific and nonspecific activation

100%
Zak-Nejmark T., Kraus-Filarska M., Malolepszy J., Jankowska R., Jutel M., Nowak I. A., Nadobna G.
|
|
vol. 48
|
issue 2
107-110
EN
Histamine is a physiological mediator which exerts both effector and regulatory functions through its receptors on various cells. The aim of the study was to investigate changes in histamine receptor expression on peripheral blood lymphocytes affected by stimulation with both specific and nonspecific stimuli. Lymphocytes were obtained from both healthy and allergic subjects. Cells were incubated with various allergens (mixed grass pollen, Lolium perenne, Dermatophagoides pteronyssinus 1, bee venom, phospholipase A2) and nonspecific (fMLP, PMA/ionomycin, LPS) stimuli. The percentage of histamine-binding cells was determined with a fluorescence microscope after incubation with histamine-fluorescein. In control subjects histamine binding after stimulation with allergens was not significantly changed. In contrast, in allergic subjects stimulation with specific allergens resulted in significantly increased histamine binding. Nonspecific stimulation caused increased histamine binding to lymphocytes in both allergic subjects and healthy controls. We conclude that specific and nonspecific activation of lymphocytes is associated with increased expression of histamine receptors.
15

Expression of naive/memory (CD45RA/CD45RO) markers by peripheral blood CD4+ and CD8+ T cells in children with asthma

100%
Machura E., Mazur B., Pieniazek W., Karczewska K.
|
|
issue 1
55-62
EN
Introduction: The role of CD4+ T cells in the immunopathogenesis of asthma is well documented. Little is known about the role of CD8+ T cells. The aim of this study was to assess peripheral blood subsets of CD4+ and CD8+ T cells expressing naive/memory markers (CD45RA+/RO+) and the activation marker (CD25+) in children with allergic asthma. Materials and Methods: Peripheral blood mononuclear cells were isolated from children with allergic asthma and healthy children. T cell subsets were analyzed by flow cytometry for the expressions of CD45RA, CD45RO, and CD25. In this study, some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls were detected. Results: The absolute number of CD8+ T cells expressing CD45RO was significantly elevated and the percentages of CD3+ T cells expressing activation marker CD25 and of CD4+ T cells expressing memory marker CD45RO were significantly lower in children with asthma compared with controls. No correlation was found between severity of asthma and peripheral blood lymphocyte subsets. Conclusions: There were some differences in the memory compartment of peripheral blood T cells between asthmatic children and healthy controls. The increase in the number of CD8+ T cells expressing the memory marker (CD45RO) in children with allergic asthma may indicate that CD8+ T cells play a role in the pathogenesis of asthma.
16

Structure of antibody and limphocytes of receptor T ? similarities and differences

88%
Madalinski K., Michalkiewicz M. J., Michalkiewicz J.
Biotechnologia
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2001
|
issue 3
71-77
EN
In this paper the structure of molecules responsible for recognition of foreign antigen is given.
17

CD80 and CD86 expression on LPS-stimulated monocytes and effect of CD80 and CD86 blockade on IL-4 and IFN-gamma production in nonatopic bronchial asthma

88%
Rutkowski R., Moniuszko T., Stasiak-Barmuta A., Kosztyla-Hojna B., Alifier M., Rutkowski K., Tatarczuk-Krawiel A.
Archivum Immunologiae et Therapiae Experimentalis
|
2003
|
vol. 51
|
issue 6
421-428
EN
CD80 and CD86 seem to play an important role in the allergen induced secretion of IL-5 and IL-13. Up till now, the expression of CD80 (B7.1) and CD86 (B7.2) on monocytes and kinetics of these molecules expression on lipopolysaccharide?stimulated monocytes in nonatopic asthma have not been defined. Using monoclonal antibodies we have compared the expression of CD80 (B7.1) and CD86 (B7.2) on monocytes of healthy persons and nonatopic asthmatic patients. We have also assessed the effect of CD80 and CD86 inactivation on interleukin (IL)-4 and interferon gamma (IFN-gamma production in nonatopic asthmatics and healthy subjects. We found that low expression of CD80 on studied monocytes (1.64+0.65 vs. 3.53+1.43%) and moderate expression of CD86 (41.25+134 vs. 49.46+11.49%) were characteristic for asthma. In nonatopic asthma patients inactivation of CD80 or CD86 blockade significantly reduced IFN-gamma production by T lymphocytes (p<0.02; p<0.03). In both studied groups anti-CD80 antibodies did not diminish T lymphocytes` production of IL-4. However anti-CD86 antibodies significantly (p<0.04) reduced the IL-4 concentration in culture supernatants. Our results confirm that both CD80 and CD86 molecules play on important role in the maintenance and amplification of inflammatory process. It suggests that in the inflammatory process that occurs in the nonatopic bronchial asthma Th1 as well as Th2 lymphocytes are equally important
18

The effect of thymic hormones/TFX/ on lymphocyte subpopulations and proliferation after maximal physical effort

88%
Tchorzewski H., Zeman K., Lewicki R., Fornalczyk-Wachowska E., Golinska A., Kantorski J., Majewska E., Pokoca L.
|
|
vol. 40
|
issue 2
157-162
EN
The studies were performed on healthy well-trained cyclists. Maximal physical exercise was performed on a Monark bicycle ergometer according to individual schemes. Heart rate amounting to about 200 bts/min and oxygen consumption stabilization were considered as criteria for maximal physical exercise. In this study we have investigated the effect of short-term stimulation of conditioned sportsmen with thymic hormones and evaluated T cell subsets, DR antigen and transferrin receptor expression as well as mitogen-induced proliferation of lymphocytes before and after maximal physical effort. The results suggest that intensive physical exercise may be responsible for transient decrease of CD4/CD8 ratio and mitogen responsiveness, and increase of mononuclear cells number bearing HLA DR + and CD71 antigens. These changes were modified by the treatment with thymic hormones.
19

Genotoxicity of the volatile anaesthetic desflurane in human lymphocytes in vitro, established by comet assay

88%
Karpinski T. M., Kostrzewska-Poczekaj M., Stachecki I., Mikstacki A., Szyfter K.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 3
319-324
EN
The aim of the present study was to estimate the genotoxicity of desflurane, applied as a volatile anaesthetic. The potential genotoxicity was determined by the comet assay as the extent of DNA fragmentation in human peripheral blood lymphocytes in vitro. The comet assay detects DNA strand breaks induced directly by genotoxic agents as well as DNA fragmentation due to cell death. Another anaesthetic, halothane, already proved to be a genotoxic agent, was used as a positive control. Both analysed drugs were capable of increasing DNA migration in a dose-dependent manner under experimental conditions applied. The results of the study demonstrated that the genotoxicity of desflurane was comparable with that of halothane. However, considering the pharmacodynamics of both drugs, the genotoxic activity of desflurane may be connected with a less harmful effect on the exposed patients or medical staff.
20

The effect of hFVIII transgene on the chromosomal aneuploidy rate in rabbits

88%
Curlej J., Parkanyi V., Bulla J., Jurcik R., Chrenek P.
Folia Biologica
|
2007
|
vol. 55
|
issue 3-4
161-164
EN
The aim of this study was to compare the chromosomal aneuploidy rate between transgenic and non-transgenic rabbits derived from the F4 generation. Chromosomal analysis was carried out on bone marrow samples of New Zealand White transgenic (carrying human factor VIII gene) and non-transgenic rabbits (F4 generation) each having a different genetic background (female no. 1-3-5 line I and female no.1-9-7 line II). C-metaphase plates were obtained from the bone marrow lymphocytes synchronized by the addition of 0.25 mug/ml colcemide. No significant difference in chromosomal aneuploidy between transgenic (61%) and non-transgenic (51.27%) rabbits of line I was observed. A higher but non-significant aneuploidy rate between transgenic and non-transgenic rabbits was found in line II, on the other hand a significant difference (P<0.05) was observed in diploidy rate. In conclusion, chromosomal aneuploidy rates in this experiment were higher than published previously in other reports.
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