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EN
The paper contains a review of references for 35 genes controlling alkaloid content, growth rhythm, branching, vegetation length, shape and color of leaves, color of flowers and seeds, seed hardiness, pod characters and disease resistance.Authors of characters and gene symbols from different research centres and from different years are given on the basis of priority.The importance of these genes for breeding improvement is discussed.Type lines for characters/genes and genotype of more important cultivars colected in the Polish narrow-leafed lupin genebank are presented.
EN
BAC (bacterial artificial chromosome) clones from the genomic BAC library of the narrow-leafed lupin (Lupinus angustifolius) were used for cytogenetic mapping of mitotic metaphase chromosomes of that species by the BAC-FISH technique. Location of the clones, together with cytogenetic markers localised earlier by FISH (fluorescence in situ hybridisation) and PRINS (primed in situ DNA labelling), was combined with computer-aided chromosome measurements, to construct the first idiogram of the narrow-leafed lupin. The chromosomes are meta- or submetacentric; the mean absolute chromosome lengths range from 1.9 mum to 3.8 mum, and mean relative lengths from 1.6% to 3.3%. Data concerning linkage of resistance to 2 fungal pathogens as well as assignment of the second linkage group to the appropriate chromosome are given for the first time.
EN
The limited gene pool used in breeding decreases the level of genetic diversity in legume cultivars. Morphological or physiological characters are not always sufficient for quick, easy and precise cultivar description. Isozyme variability of 33 commercial Polish pea cultivars was analysed. The level of allozyme polymorphism discovered was high enough for the identification of all cultivars within two groups: white flowering peas for human consumption and colored flowering peas for fodder. The range of RAPD marker polymorphism among three lupin crops (white lupin, narrow-leafed lupin and yellow lupin) was tested. It was possible to identify each of four white lupin cultivars by means of bands generated by two primers. Seven narrow-leafed lupin cultivars were distinguished using three other primers. Testing of RAPD marker polymorphism, supplemented in some cases with observations of seed coat color genes, allowed to identify all 12 yellow lupin cultivars. Thirteen field bean cultivars were tested by isozyme variability and RAPD polymorphism observations. Among 15 enzyme systems investigated, 10 showed a high level of inter- and intracultivar polymorphism but the range of allozyme variability discovered was useless for cultivar identification. All cultivars analysed could be distinguished by a combination of RAPD markers amplified using two primers.
EN
The pollen grain germination and the growth of pollen tubes after intra and interspecific reciprocal polination of Lupinus albus L., Lupinus mutabilis Sweet. and Lupinus angustifolius L. were examined.Results of microscopic observations of pollen grain germination and pollen tube growth enable to deny the existence of certain barriers that prevent fertilization after pollination of L.albus flowers with pollen of L.angustifolius and vice versa, as well as after pollination of L.mutabilis flowers with pollen of L.angustifolius and vice versa.Some delay in pollen grain germination and a slower growth of pollen tubes were observed in all combinations, as related to intraspecific ones, though in case of each intrspecific combination pollen tubes reached the ovary.Moreover, a different level of pollination grain germination and pollen tube growth, depending on the direction of pollination, were found.The noted pollination efficiency may generally considered to be low.
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