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Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides)

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Yue Y.-W., Long H., Liu Q., Wei Y.-M., Yan Z.-H., Zheng Y.-L.

Journal of Applied Genetics

2005

vol. 46

issue 4

349-355

Three low-molecular-weight glutenin subunit (LMW-GS) genes, designated LMW-Td1, LMW-Td2 and LMW-Td3, were isolated from wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of common wheat (T. aestivum). The complete nucleotide sequence lengths of LMW-Td1, LMW-Td2 and LMW-Td3 are 858, 900 and 1062 bp, respectively. LMW-Td1 and LMW-Td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas LMW-Td2 is a putative pseudogene due to the presence of 3 inframe stop codons in its C-terminal domain. The deduced protein sequences of the 3 genes share the same typical polypeptide structures with known LMW-GS genes containing 8 cysteines in the mature protein domains. LMW-Td1 was clearly distinguished from all known LMW-GS genes, and considered as a novel LMW-GS gene. Two hydrophobic motifs (i.e. PIIIL and PVIIL) were observed in the repetitive domain of LMW-Td3. Sequence comparison indicates that sequences of the 3 LMW-GS genes from this study are strongly similar to known LMW-GS genes. Our phylogenetic analysis suggests that LMW-Td1 and LMW-Td2 are homologous with genes on chromosome 1A, and LMW-Td3 is closely related to genes on chromosome 1B.

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1 Journal of Applied Genetics

1 2005

1 Liu Q.

1 Long H.

1 Wei Y.-M.

1 Yan Z.-H.

1 Yue Y.-W.

1 Zheng Y.-L.

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Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides)