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Lectin histochemistry and alkaline phosphatase activity in the pia mater vessels of spontaneously hypertensive rats (SHR)

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Szumanska G., Gadamski R.

Neuropatologia Polska

1992

vol. 30

issue 2

99-110

Some lectins were used to study the localization of sugar residues on the endothelial cell surface in the pia mater vessels of control (WKY) and hypertensive rats (SHR). The lectins tested recognized the follwing residues: beta-D-galactosyl (Ricinus communis agglutinin 120, RCA-1), alpha-L-fucosyl (Ulex europaeus agglutinin, UEA-1), N-acetylglucosaminyl and sialyl (Wheat germ agglutinin, WGA), N-glocyl-neuraminic acid (Limax flavus agglutinin, LFA) and N-acetyl-D-galactosaminyl (Helix pomatia agglutinin, HPA). Several differences were revealed in the presence of sugar receptors on the surface of endothelial cells between the control and the hypertensive rats. Our studies showed also differences in the localization of the tested glycoconjugates between pial capillaries, small,medium-size and large pial arteries. The histochemical evaluation of alkaline phosphatase revealed an increased activity of the enzyme in the pial vessels of SHRs as compared with control rats, with a similar localization of the enzyme activity. Some differences in the distribution of lectin binding sites and alkaline phosphatase activity could be associated with the different functions of particular segments of the pial vascular network.

How endothelial cell organo-specificity mediates circulating cell homing

100%

Kieda C.

Archivum Immunologiae et Therapiae Experimentalis

2003

vol. 51

issue 2

81-89

Normal and transformed cells are homing from the circulation into tissues in a very selective way thanks to highly complex molecular mechanisms that govern cell-to-cell interactions and drive the homing of circulating cells to be achieved properly. Because this is characterised by a resulting high selectivity, it constitutes a template for targeted drug-, gene- or cell-therapy strategies. Designing a mimetic-based therapy requires the identification of the responsible selective molecules but also, their mechanisms of action and interactions with their ligands, together with their biological modulation and regulation. This homing/invasion event happens to be decisive at the level of the endothelium that lines the vessel walls. Since cell-to-cell interactions mean a double recognition process, this review will illustrate the part played by the endothelial cells (EC) and their adhesion molecules: the protein as well as the glycan part point of view, the chronology and environmental modulation of EC adhesion molecules expression. These characteristics should provide keys to understand the resulting overall specificity of cell localisation. Taking into account the cytokine microenvironment, it was recently documented a fundamental role for locally secreted chemokines which act through their restricted presentation by endothelial cells. As such, chemokines contribute to illustrate the concept of endothelial organo-specificity which is approached here uncovering the role of glycoconjugates signalling as the hallmark of refined cellular recognitions and discussed, in the context of potential drug design against site-directed diseases as metastases, inflammatory leukocytes recruitment, tumour/inflammation-induced angiogenesis.

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1 Archivum Immunologiae et Therapiae Experimentalis

1 Neuropatologia Polska

1 2003

1 1992

1 Gadamski R.

1 Kieda C.

1 Szumanska G.

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Lectin histochemistry and alkaline phosphatase activity in the pia mater vessels of spontaneously hypertensive rats (SHR)

How endothelial cell organo-specificity mediates circulating cell homing