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A kinetic method for para-nitrophenol determination based on its inhibitory effect on the catalatic reaction of catalase

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Mureşanu C., Copolovici L., Pogącean F.

Open Chemistry

2005

vol. 3

issue 4

592-604

The inhibitory effect of para-nitrophenol on the catalytic reaction of catalase was investigated. Michaelis-Menten kinetic parameters were determined from Lineweaver-Burk plots obtained in the absence or in the presence of the inhibitor. The inhibitor pattern, revealed by the Lineweaver-Burk plots, suggested a fully mixed inhibition mechanism. Spectrophotometric monitoring of the indicator reaction: $$H_2 O_2 \xrightarrow{{catalase,para - nitrophenol}}H_2 O + \tfrac{1}{2}O_2 $$ in conjunction with initial rate measurements was employed for the kinetic determination of the inhibitor. Calibration plots of initial rate vs. para-nitrophenol concentration were linear in the concentration range 0.9·10−5–2.5·10−5 mol/L and the detection limit was 3·10−6 mol/L (417 μg/L) para-nitrophenol. Interferences from other phenolic compounds like orto-cresole, meta-and orto-nitrophenol were observed.

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1 Open Chemistry

1 2005

1 Copolovici L.

1 Mureşanu C.

1 Pogącean F.

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A kinetic method for para-nitrophenol determination based on its inhibitory effect on the catalatic reaction of catalase