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Biosynthesis of laccase from Cerrena unicolor: kinetic aspects of fungal growth and enzyme production

100%
Antecka A., Bizukojc M., Ledakowicz S.
Biotechnologia
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2008
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issue 2
90-101
EN
In spite of various possible industrial applications of laccase produced by wood-rotting basidiomycetes, the investigations concerning kinetics of the fungal growth are still limited. Thus, the aim of this paper was to describe the growth kinetics of white-rot fungus Cerrena unicolor and the synthesis of its secondary metabolite, laccase. Glucose was used as a carbon source in the cultivation and L-asparagine as a nitrogen source. The production of the enzyme appeared to be non-growth associated since it is secreted concomitantly to biomass decay. The formation of laccase occurred to be repressed by glucose, but there was no such strong impact of organic nitrogen content. However, the amount of biomass in the system was influenced by both asparagine and glucose concentration in the medium.
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Electrophysiological description of mechanisms determining synaptic transmission and its modulation

75%
Mozrzymas J. W.
Acta Neurobiologiae Experimentalis
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2008
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vol. 68
|
issue 2
256-263
EN
Signal integration in neurons is a complex process that depends on e.g. the kinetics of synaptic currents, distribution of synaptic connections as well as passive and excitatory membrane properties. The time course of synaptic currents is largely determined by the kinetics of the postsynaptic receptors and the time course of synaptic neurotransmitter concentration. The analysis of current responses to rapid agonist applications provides the means to study the ligand-gated receptor gating but experimentally based estimation of neurotransmitter transient at central synapses was an important challenge during the last decade. Both theoretical as well as experimentally based approaches indicated that synaptic agonist transient is very brief, implying that the activation of postsynaptic receptors occurs in conditions of extreme non-equilibrium. Such a dynamic pattern of activation of postsynaptic receptors has a crucial impact not only on the kinetics of synaptic currents but also on their susceptibility to pharmacological modulation.
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