Results found: 3

Number of results on page

Search results

Search:
in the keywords: KERATINOCYTE

Sort By:

Limit search:

In vitro culture of human epithelial cells on a modified xenogenic collagen support

100%

Lesiak-Cyganowska E., Sladowski D., Jankowska E., Komender J.

Archivum Immunologiae et Therapiae Experimentalis

2001

vol. 49

issue 3

253-259

Human epithelial cells (HeLa, HaCaT, NHK) were cultured in vitro on chemically modified collagen membranes. Adhesion to the support was measured by estimation of the percentage of adhering 51Cr-labeled cells. Proliferation was estimated with the XTT test. Morphological observations of cells growing on HCl-treated collagen were performed using histological and electron microscopic techniques. HCl and trypsin-modified xenogenic collagen was found to be a good support for human cells in vitro. EDTA-incubated collagen enhanced neither adhesion nor proliferation. The best adhesion and proliferation were found on HCl-treated collagen, depending, however, on the kind of cells.

Pathogenetic mechanisms of atopic dermatitis

88%

Pastore S., Giustizieri M. L., Mascia F., Giannetti A., Girolomoni G.

vol. 48

issue 5

497-504

Atopic dermatitis (AD) is a chronic inflammatory disease which results from complex interactions between genetic and environmental mechanisms. An altered lipid composition of the stratum corneum is responsible of the xerotic aspect of the skin, and determines a higher permeability to allergens and irritants. Keratinocytes of AD patients exhibit a propensity to an exaggerated production of cytokines and chemokines, a phenomenon that can have a major role in promoting and maintaining inflammation. Specific immune responses against a variety of environmental allergens are also implicated in AD pathogenesis with a bias towards Th2 immune responses. In particular, dendritic cells expressing membrane IgE receptors, play a critical role in the amplification of allergen-specific T cell responses. Cross-linkage of specific IgE receptors on dermal mast cells provokes release and synthesis of a vast series of mediators. Following their recruitment and activation into the skin, eosinophils are also thought to contribute relevantly to tissue damage. Thus, a complex network of cytokines and chemokines contributes to establish a local milieu that favors the permanence of inflammation in AD skin.

Comparison of proliferation and motile activity between human keratinocytes isolated from skin and oral muco

88%

Drukala J., Zarzecka J., Gojniczek K., Waligorska A., Zapala J., Korohoda W.

Folia Biologica

2005

vol. 53

issue 1-2

21-28

Objective: Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. For healing of skin wounds the skin keratinocytes can be replaced by oral mucosa epithelial cells grown in vitro. The presented experiments were carried out in order to compare the proliferation, morphology, and migration between human keratinocytes isolated from human skin and oral mucosa. Materials and methods: Human epidermal and oral mucosa keratinocytes from primary culture were used in all experiments. Cell motility and shape were determined using computer-aided methods. Results and conclusions: It was demonstrated that although both cell types exhibit the same typical epithelial morphology, oral mucosa keratinocytes locomote significantly faster than skin keratinocytes. They also differ in proliferation activity. Oral mucosa keratinocytes exhibited faster growth and different actin cytoskeleton organisation than skin keratinocytes under in vitro conditions. Autologous oral mucosa keratinocytes may be expanded in vitro and used for skin wound healing in vivo.

Refine search results

2 Archivum Immunologiae et Therapiae Experimentalis

1 Folia Biologica

1 2005

1 2001

1 2000

1 Drukala J.

1 Giannetti A.

1 Girolomoni G.

1 Giustizieri M. L.

1 Gojniczek K.

1 Jankowska E.

1 Komender J.

1 Korohoda W.

1 Lesiak-Cyganowska E.

1 Mascia F.

1 Pastore S.

1 Sladowski D.

1 Waligorska A.

1 Zapala J.

1 Zarzecka J.

Search results

In vitro culture of human epithelial cells on a modified xenogenic collagen support

Pathogenetic mechanisms of atopic dermatitis

Comparison of proliferation and motile activity between human keratinocytes isolated from skin and oral muco