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1

Introgression of Brassica napus genome into restorer lines for CMS ogura estimated with the use of molecular markers

100%
Poplawska W., Bartkowiak-Broda I.
Biotechnologia
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2001
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issue 1
112-118
EN
The object of research were double low restorer lines of CMS ogura. The restorer gene was introgressed into rapeseed genotype from radish (Raphanus sativus) through intergeneric hybridisation. The obtained recombinants of rapeseed with the restorer gene comprise a DNA fragment originating from radish, which is bigger than the locus of the restorer gene. It disturbs the first meiotic behaviour in PMC. In addition, it has an impact on the fertility and yielding ability of restorer lines. Moreover, the restorer gene in the obtained recombinant is tightly linked with a gene responsible for high content of undesirabled compounds in seeds - glucosinolates. Elimination of the unnecessary DNA fragment is performed by backcrosses with double low lines of winter rapeseed. The changes in rapeseed genome which are a result of backcrosses are investigated by the use of molecular and isozyme markers. Key words: Brassica napus, restorer gene, introgression, molecular and isozyme markers. Adres do korespondencji:
2

Aegilops-rye amphiploids and substitution rye used for introgression of genetic material into rye (Secale cereale L.)

88%
Apolinarska B., Wisniewska H., Wojciechowska B.
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vol. 51
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issue 4
413-420
EN
The valuable genes of Aegilops biuncialis, Ae. ovata, Ae. kotschyi, and Ae. variabilis were transferred to rye, by crossing Aegilops-rye amphiploids with tetraploid and diploid substitution rye. The C-banded karyotype of the BC1 and BC2 generations of amphiploids with 4x substitution rye and BC1 with 2x substitution rye showed great variation in chromosome number and composition. In the BC1 generation of amphiploids with 4x and 2x substitution rye, seed set success rate and germination rate varied depending on origin. However, plant sterility in all cross combinations of amphiploids with 4x and 2x substitution rye resulted in their elimination from further experiments in the BC3 and BC2 generations, respectively. In backcrosses of 4x substitution rye with amphiploids Ae. variabilis x rye 4x, fertile 4x rye plants containing Aegilops chromatin were produced in the BC2 generation.
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