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1

Present status of biotechnology of rye (Secale cereale L.)

100%
Bolesta E., Rybczynski J. J.
Biotechnologia
|
2001
|
issue 4
55-73
EN
This review paper covers the results of tissue culture and biotechnology of rye (Secale cereale L.) published between 1990 and 2000. The following subjects were raised: somatic embryogenesis, haploid production, intergenetic hybridisation and transformation. Upon conclusion of all the results published to date, we may say that the progress of biotechnology in the case of rye is very limited in comparison to other cereals. However, a lot of work and efforts were involved to obtain the aforementioned result. Taking into account the progress in plant genetics and molecular biology, the authors deeply believe that the New Millennium will bring a brake-through in rye biotechnology.
2

Production and morphology of the hybrids Aegilops kotschyi ? Secale cereale and Ae. biuncialis ? S. cereale

100%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
279-285
EN
Hybrids between Aegilops kotschyi and Ae. biuncialis with Secale cereale were synthesized. Five Ae. kotschyi and four Ae. biuncialis accessions, as well as one inbred and four self-compatible forms of Secale cereale were used for crossing. The hybrids were produced directly from cultured embryos or through embryo callus culture. Sixty hybrids, 11 involving Ae. kotschyi and 49 Ae. biuncialis, had a stable somatic chromosome number 2n = 3x = 21. The plants showed good vegetative vigour and tillering capacity. Morphologically the hybrids were intermediate between their parents and completely sterile. In vitro propagation of Ae. kotschyi and Ae. biuncialis ? S. cereale hybrids revealed that their capacity for callus production and plantlet regeneration ? varies.
3

Chromosome pairing in triploid intergeneric hybrids of Festuca pratensis with Lolium multiflorum, revealed by GISH

100%
Kosmala A., Zwierzykowska E., Zwierzykowski Z.
Journal of Applied Genetics
|
2006
|
vol. 47
|
issue 3
215-220
EN
Genomic in situ hybridisation (GISH) was used to reveal chromosome pairing in two partly fertile, triploid (2n = 3x = 21) hybrids obtained by crossing the diploid (2n = 2x = 14) Festuca pratensis Huds. (designated FpFp), used as a female parent, with the autotetraploid (2n = 4x = 28) Lolium multiflorum Lam. (designated LmLmLmLm), used as a male parent. The pattern of chromosome pairing calculated on the basis of the mean values of chromosome configurations identified in all 100 PMCs analysed, was: 0.71I Lm + 2.24I Fp + 2.18II Lm/Lm + 0.54II Lm/Fp + 4.18III Lm/Lm/Fp. A relatively high number of Lm/Lm bivalents and Fp univalents, and a low number of Lm/Fp bivalents and Lm univalents indicated that the pairing was preferential between L. multiflorum chromosomes. Other observations regarding chromosome pairing within the Lm/Lm/Fp trivalents also confirmed this preferential pairing in the analysed triploids, as the Fp chromosome was not randomly located in the chain- and frying-pan-shaped trivalents. The similarities and differences in chromosome pairing at metaphase I and the level of preferential pairing between Lolium chromosomes in the different triploid Lolium-Festuca hybrids are discussed.
4

Interspecific and intergeneric hybrids of the Lolium-Festuca complex obtained in Poland in the years 1964-1994 and maitained in the collection at the Institute of Plant Genetics in Poznan

86%
Zwierzykowski Z.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 1
79-100
EN
In the years 1964-1994 an extensive programme of wide hybridization within the Lolium-Festuca complex was carried out in Poland.Six Lolium (ryegrass) and five Festuca (fescue) species at differnt ploidy level were used for crosses.Hybrids were obtained from 72 cross combinations.This article presents a complete list of Folium-Festuca hybrids obtained in Poland in the years 1964-1994 and maintained in the collection of Institute of Plant Genetics in Poznan.The available literature concerning these hybrids is cited.
5

Embryo-callus regenerated hybrids and amphidiploids of barley cultivars x rye inbred lines

86%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 4
313-323
EN
In the presented hybridization programme of barley cultivars and rye inbred lines including 48 cross combinations the seed set ranged from 3.13 to 92.98%, while embryos were formed in 0.74 to 36.36% in successful pollinations.Sixty five plants were generated by embryo callus culture and one - by embryo culture without callus formation.The hybrids had somatic chromosome numbers 2n=14 (60 plants) and 2n=28 (6 plants).Plants obtained vie embryo callus culture showed good vegetative vigour and well-developed root system.Spike morphology of all plants resambled that of rye.Meiosis in 17 diploids showed 0.13-0.63 barley-barley and rye-rye bivalents with a chiasma frequency of 0.14-0.69 per cell. The hetromorphic bivalent-like configurations occured in five plants in 0.01-0.02 per cell.The amphidiploids had 7.79-10.71 barley-barley and rye-rye bivalents with the chiasm frequency of 9.36-17.75 per cell.All plants, with 14 and 28 chromosomes, were completly sterile both in backcrosses and when selfed.
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