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1

The use of laser light for obtaining barley haploids by Hordeum bulbosum method

100%
Rybinski W., Surma M., Adamski T.
Biotechnologia
|
2001
|
issue 1
143-147
EN
The effect of helium-neon laser with wavelength of 632 nm on seed setting in a cross of Hordeum vulgare x H. bulbosum was studied. The seeds before sowing as well as immature embryos were irradiated with laser light. Material not irradiated with laser beams constituted the control. It was shown that stimulative dose of laser beams increased the number of seeds/100 pollinated florets in comparison with the control combination. After laser treatment, the seed setting of the line HG156, and cultivars Vada and Apex was higher by 13.0, 7.9 and 3.2%, respectively. The number of obtained haploids/100 cultured embryos irradiated with laser light was also higher in comparison with the control by 1.8, 0.9 and 10.8%, respectively. The obtained results show that treatment with laser beams was more effective at the first step of haploid production (seeds/100 florets) than at the next step (haploids/100 embryos cultured). From the practical point of view, better results can be obtained by irradiation of seeds before sowing than by irradiation of immature embryos. Cultivar Apex with a positive reaction on irradiation of embryos was an exception here.
2

Evaluation of crossability of Cuphea lanceolata and C.viscosissima based on pollen tube rowth and seed set

86%
Wojciechowski A., Olejniczak J., Adamska E.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 3
277-384
EN
Many species of Cuphea genus show promise as rich natural sources of valuable seed oils for commercial utility.Among them are C.lancelota and C.viscosissima which seed oils contain mediumm-chain fatty acids.Recoprocal crosses were made between species to assess their usefulnes for Cuphea breeding programme.The degree of crossability between C.lanceolata and C.viscosissima was determinated by pollen grain germinatin and pollen tube growth observed using fluorescence microscopy in addition to capsule formation and seed number per capsule.Treatment of the pollen grain with a laser microbeam reduced the number of germinated pollen grains, tubes growth in the pistil and seed set when C viscisissima plants were used in the crosses as a female form.In the cross C.lanceolata (female form) x C viscosissima the pollen treatment with a laser microbeam improved pollen tube growth but reduced seed set.Crossability evaluated on the basis of pollen tube growth and seed set showed unilateral intraspecific incompatibility in crosses involving C.lanceolata plants as a female form.
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