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Application of UV detection in HPLC in the total antioxidant potential assay

100%
Wantusiak P., Głód B.
Open Chemistry
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2012
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vol. 10
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issue 6
1786-1790
EN
HPLC has been already used for the TAP estimation. Phenylalanine, salicylic, p-hydroxybenzoic (pHBA) or terephthalic (TPA) acids have been used as sensor compounds. Products of their reaction with the hydroxyl radicals, generated in the Fenton-like reaction, were analyzed using electrochemical or fluorescence detection. This paper describes the TAP assay based on the hydroxyl radicals reaction with pHBA, reversed-phase-HPLC separation and UV photometric detection. The elaborated assay has been used to evaluate TAP values of some apiculture products. [...]
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Total Phenolic, Flavonoid Contents and Antioxidant Activities of Honey and Propolis Collected from the Region of Laghouat (South of Algeria)

86%
Bakchiche B., Habati M., Benmebarek A., Gherib A.
World News of Natural Sciences
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2017
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vol. 11
91-97
EN
The aim of this study was to determine total phenolic and flavonoid contents and to evaluate the antioxidant activities of two honeys and one propolis sample, collected from the region of Laghouat (South of Algeria). Total phenolic content were determined by using Folin-Ciocalteu reagent as gallic acid equivalent, while flavonoids content was assessed by means of the AlCl3 method as Rutin equivalent. Antioxidant activities of the honeys and propolis were examined by two different methods, namely, scavenging of free radical 2,2-diphenyl-1-picrylhydrazyl and reducing power. The antioxidant activities were compared with standard antioxidants such as Ascorbic acid, BHT and Trolox. The highest level of phenolic was 2385 mg Gallic acid per 100g sample, the highest level of flavonoid was 379 mg Rutin per 100g sample, while the highest protein content was 1177 mg per 100g sample, DPPH (0.026 mg/ml) and TEAC (0.0015) were detected especially in the propolis sample, indicating that it holds good antioxidant properties. A strong positive correlation was found between phenolics, flavonoids, DPPH and TEAC, indicating that in addition to total phenolic content, flavonoid and protein concentrations are good indicators of the antioxidant potential of propolis and honey.
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Pollen analysis and heavy metals detection in honey samples from southern Nigeria

58%
Adekanmbi O. H., Walter O. J., Ikegbunam N. C.
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vol. 26
176-190
EN
Pollen analytical study of two locally produced honey samples collected from Akwa Ibom and Cross River State, Southern Nigeria were carried out in order to ascertain the preferentially foraged plants by honey bees, as well as the concentration of heavy metals. Samples were subjected to standard laboratory procedures using an acetolysis mixture (pollen analysis) and atomic absorption spectrometry (heavy metals). The results from the analysis revealed 32 taxa belonging to 17 botanical families. The number of pollen grains in the honey samples varied significantly (between 4,887 - 12,755 pollen grains), indicating their richness in pollen grains. 12 elements - Ni, Cu, Zn, Se, Br, K, Ca, Ti, Cr, Mn, Rb, and Fe were detected. Potassium had the highest concentration, followed by iron, calcium, titanium, zinc, copper, chromium, nickel, among others. What is more, in this study, some important honey plants: Spondias mombin, Daniella oliveri, Manihot esculenta, Treculia africana, Syzigium guineensis, Diospyros mespiliformis, Parkia biglobosa, Terminalia superba, Senna hirsuta, among others were found to be predominant and have been identified to be characteristic of the vegetation typical of Southern Nigeria. With regard to honey, this study gives an indication of the geographical and botanical origins, as well as types, source, and degree of contamination, and also an overall measure of honey purity. Such information, when displayed, can help consumers make informed decision when purchasing honey and will also help beekeepers to avoid possible contamination. Moreover, it will assist regulatory agencies in taking proper measures for environmental and consumer protection, since the concentration of heavy metals in honey is influenced by environmental pollution.
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