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Biotechnologia
|
2006
|
issue 2
166-188
EN
The paper reviews the aspects of physiology and biochemistry of microbial biodegradation of the main components of petroleum oil: alkanes (larger than methane), alkenes, alkines, cyclic, polycyclic, aromatic and polyaromatic hydrocarbons. It focuses on aerobic degradation pathways. The review provides key information prepared on the basis of more than 30 years of research on microbial degradation of hydrocarbons. The areas discussed include new pathways of biodegradation of branched-chain alkanes, cyclic and polyaromatic hydrocarbons which have been discovered during the past few years. Special attention was paid to oxygenases ? enzymes initiating aerobic metabolism of hydrocarbons and phenomenon of co-oxidation which enables assimilation of most recalcitrant components of crude oil.
EN
Pseudomonas aeruginosa and Pseudomonas putida, as Grampositive and Bacillus subtilis and Bacillus licheniformis as Gramnegative strains were used as biological agents in biodegradation process of model mixture of hydrocarbons alone. Furthermore in the presence of the synthetic emulsifiers of polyoxyethylenated alcohol and alkylglucoside derivatives, natural emulsifier of rhamnolipid derivatives and anionic surfactants were used.
EN
Microorganisms present in water samples from various industrial effluents were analysed for their resistance to lead, chromium, and cadmium. The ability of these microorganisms to grow on or metabolize toxic hydrocarbons and pesticides was also checked. Microorganisms in samples from the steel and tanning industries were generally resistant to metal ions but were not capable of metabolizing toxic hydrocarbons. Conversely, microorganisms found in samples of pesticide and from the chemical industry were capable of metabolizing hydrocarbons and pesticides but were not much resistant to metal ions. Microorganisms from effluents of the paint industry and urban wastes were resistant to lead. A correlation between the population of microorganisms and the type of pollution was observed. Indigenous microorganism could be regarded as indicators of pollution and be used in various operations to resist, process, metabolize, and detoxify toxic industrial wastes.
EN
The interest in the use of metal porphyrins in biomimetic catalysis has been constantly increasing during the last twenty years, as the enzymology studies were showing the role of this complexes as prosthetic groups of cytochromes P-450, peroxidases and catalases. In particular, a number of research groups have focused their interest on the ability of metal porphyrins to catalyze redox processes on organic compounds under mild conditions, both for converting these compounds into more usuful products and for purifying industrial effluents. The aim of this review is to highlight the efforts of researchers in utilising metalloporphyrins for economically attractive processes. Interesting reactions are allylic hydroxylations of cheap and readily available precursor monoterpenes (like limonene, pinene) for potential production of valuable natural flavour and fragrance compounds, selective oxidation of alkyl chains of the alkanes, regioselective oxidation of cyclic hydrocarbons (eg. cycloheksan), steroids, as well as reduction of the halogenated alkanes.
EN
Three plant species belonging to Compositae, Chenopodiceae and Potamogetonaceae were collected from Burullus Lake.Hydrocarbon, sterol and fatty acid composition of each species were investigated and identified quantitatively using GLC technique.The result demonstrated that, the unasaponifiable matter of Inula erithmoides Halimione portulacoides and Potamogeton pectinatus was composed of, respectively 74.7% hydrocarbons and 25.3% sterol compounds; 73.6% hydrocarbons and 26.5% sterol compounds;100% hydrocarbons.Two sterols, namely cholesterol and stigmasterol, were detected in extracts of Inula erithmoides and Halimione portulacoides but at different concentrations.The analysis of saturated and unsaturated fatty acids indicated that the species studied differed in the number and concentration of fatty acids.
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