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The influence of calyculin A on lymphocytes in vitro

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Kowalska A., Srebniak M., Wawrzkiewicz A., Kaminski K.
Journal of Applied Genetics
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2003
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vol. 44
|
issue 3
413-418
EN
Human lymphocytes were cultured in vitro and treated with calyculin A. The aim of this work was to estimate the influence of calyculin A on chromosome morphology and banding patterns. It was also interesting whether calyculin A treatment is useful in cytogenetic analysis of human karyotype. We proved that calyculin A induces chromosome condensation in lymphocytes and raises the mitotic index significantly. Moreover, calyculin A does not influence the banding patterns. Therefore it is concluded that calyculin A can be clinically useful for human karyotyping.
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